Abstract

Enzymatic catalysis in living cells enables the in-situ detection of cellular metabolites in single cells, which could contribute to early diagnosis of diseases. In this study, enzyme is packaged in amorphous metal-organic frameworks (MOFs) via a one-pot co-precipitation process under ambient conditions, exhibiting 5–20 times higher apparent activity than when the enzyme is encapsulated in corresponding crystalline MOFs. Molecular simulation and cryo-electron tomography (Cryo-ET) combined with other techniques demonstrate that the mesopores generated in this disordered and fuzzy structure endow the packaged enzyme with high enzyme activity. The highly active glucose oxidase delivered by the amorphous MOF nanoparticles allows the noninvasive and facile measurement of glucose in single living cells, which can be used to distinguish between cancerous and normal cells.

Details

Title
Packaging and delivering enzymes by amorphous metal-organic frameworks
Author
Wu, Xiaoling 1 ; Yue, Hua 2 ; Zhang, Yuanyu 3 ; Gao, Xiaoyong 2   VIAFID ORCID Logo  ; Li, Xiaoyang 3 ; Wang, Licheng 3 ; Cao, Yufei 3 ; Hou, Miao 3 ; An, Haixia 2 ; Zhang, Lin 4 ; Li, Sai 5   VIAFID ORCID Logo  ; Ma, Jingyuan 6 ; He, Lin 6 ; Fu, Yanan 6 ; Gu, Hongkai 7 ; Lou, Wenyong 8   VIAFID ORCID Logo  ; Wei, Wei 2   VIAFID ORCID Logo  ; Zare, Richard N 9   VIAFID ORCID Logo  ; Ge, Jun 10   VIAFID ORCID Logo 

 Key Laboratory of Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing, China; School of Food Science and Engineering, South China University of Technology, Guangzhou, China 
 State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, China 
 Key Laboratory of Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing, China 
 Department of Biochemical Engineering and Key Laboratory of Systems Bioengineering of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, China 
 Key Laboratory of Protein Science, Ministry of Education, School of Life Sciences, Tsinghua University, Beijing, China; Tsinghua-Peking Joint Center for Life Sciences, Beijing, China; Beijing Advanced Innovation Center for Structural Biology, Beijing, China 
 Shanghai Synchrotron Radiation Facility, Zhangjiang Lab, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai, China 
 State Key Laboratory of Superhard Materials, Jilin University, Changchun, China 
 School of Food Science and Engineering, South China University of Technology, Guangzhou, China 
 Department of Chemistry, Fudan University, Jiangwan Campus, Shanghai, China 
10  Key Laboratory of Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing, China; Biopharmaceutical and Health Engineering Division, Tsinghua Shenzhen International Graduate School, Shenzhen, China 
Pages
1-8
Publication year
2019
Publication date
Nov 2019
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2314540472
Copyright
© 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.