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Abstract
Field cress (Lepidium campestre L.), despite its potential as a sustainable alternative oilseed plant, has been underutilized, and no prior attempts to characterize the genome at the genetic or molecular cytogenetic level have been conducted. Genetic maps are the foundation for anchoring and orienting annotated genome assemblies and positional cloning of candidate genes. Our principal goal was to construct a genetic map using integrated approaches of genetic, comparative and cytogenetic map analyses. In total, 503 F2 interspecific hybrid individuals were genotyped using 7,624 single nucleotide polymorphism markers. Comparative analysis demonstrated that ~57% of the sequenced loci in L. campestre were congruent with Arabidopsis thaliana (L.) genome and suggested a novel karyotype, which predates the ancestral crucifer karyotype. Aceto-orcein chromosome staining and fluorescence in situ hybridization (FISH) analyses confirmed that L. campestre, L. heterophyllum Benth. and their hybrids had a chromosome number of 2n = 2x = 16. Flow cytometric analysis revealed that both species possess 2C roughly 0.4 picogram DNA. Integrating linkage and comparative maps with cytogenetic map analyses assigned two linkage groups to their particular chromosomes. Future work could incorporate FISH utilizing A. thaliana mapped BAC clones to allow the chromosomes of field cress to be identified reliably.
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1 Swedish University of Agricultural Sciences, Department of Plant Breeding, Alnarp, Sweden (GRID:grid.6341.0) (ISNI:0000 0000 8578 2742)
2 University of Silesia, Department of Plant Anatomy and Cytology, Katowice, Poland (GRID:grid.11866.38) (ISNI:0000 0001 2259 4135)
3 Mirpur University of Science and Technology (MUST), Mirpur AJK, Pakistan (GRID:grid.449138.3); University of Birmingham, School of Biosciences, Birmingham, United Kingdom (GRID:grid.6572.6) (ISNI:0000 0004 1936 7486)
4 University of Birmingham, School of Biosciences, Birmingham, United Kingdom (GRID:grid.6572.6) (ISNI:0000 0004 1936 7486)
5 UTP University of Science and Technology, Laboratory of Molecular Biology and Cytometry, Department of Agricultural Biotechnology, Bydgoszcz, Poland (GRID:grid.412837.b) (ISNI:0000 0001 1943 1810)
6 Agriculture and Agri-Food Canada, Saskatoon, Canada (GRID:grid.55614.33) (ISNI:0000 0001 1302 4958)
7 Swedish University of Agricultural Sciences, Department of Animal Breeding and Genetics, Uppsala, Sweden (GRID:grid.6341.0) (ISNI:0000 0000 8578 2742)