Abstract

Human herpesvirus 6B (HHV-6B) belongs to the β-herpesvirus subfamily of the Herpesviridae. To understand capsid assembly and capsid-tegument interactions, here we report atomic structures of HHV-6B capsid and capsid-associated tegument complex (CATC) obtained by cryoEM and sub-particle reconstruction. Compared to other β-herpesviruses, HHV-6B exhibits high similarity in capsid structure but organizational differences in its CATC (pU11 tetramer). 180 “VΛ”-shaped CATCs are observed in HHV-6B, distinguishing from the 255 “Λ”-shaped dimeric CATCs observed in murine cytomegalovirus and the 310 “Δ”-shaped CATCs in human cytomegalovirus. This trend in CATC quantity correlates with the increasing genomes sizes of these β-herpesviruses. Incompatible distances revealed by the atomic structures rationalize the lack of CATC’s binding to triplexes Ta, Tc, and Tf in HHV-6B. Our results offer insights into HHV-6B capsid assembly and the roles of its tegument proteins, including not only the β-herpesvirus-specific pU11 and pU14, but also those conserved across all subfamilies of Herpesviridae.

Details

Title
Atomic structure of the human herpesvirus 6B capsid and capsid-associated tegument complexes
Author
Zhang, Yibo 1   VIAFID ORCID Logo  ; Liu, Wei 2 ; Li, Zihang 3   VIAFID ORCID Logo  ; Kumar, Vinay 3   VIAFID ORCID Logo  ; Alvarez-Cabrera, Ana L 3 ; Leibovitch, Emily C 4 ; Cui, Yanxiang 5   VIAFID ORCID Logo  ; Ye Mei 6   VIAFID ORCID Logo  ; Guo-Qiang Bi 7   VIAFID ORCID Logo  ; Jacobson, Steve 4 ; Z Hong Zhou 3   VIAFID ORCID Logo 

1  Center for Integrative Imaging, Hefei National Laboratory for Physical Sciences at the Microscale, and School of Life Sciences, University of Science and Technology of China (USTC), Hefei, Anhui, China; California NanoSystems Institute, University of California, Los Angeles (UCLA), Los Angeles, CA, USA 
2  Center for Integrative Imaging, Hefei National Laboratory for Physical Sciences at the Microscale, and School of Life Sciences, University of Science and Technology of China (USTC), Hefei, Anhui, China; California NanoSystems Institute, University of California, Los Angeles (UCLA), Los Angeles, CA, USA; Department of Microbiology, Immunology and Molecular Genetics, UCLA, Los Angeles, CA, USA; State Key Laboratory of Precision Spectroscopy, School of Physics and Electronic Science, East China Normal University (ECNU), Shanghai, China 
3  California NanoSystems Institute, University of California, Los Angeles (UCLA), Los Angeles, CA, USA; Department of Microbiology, Immunology and Molecular Genetics, UCLA, Los Angeles, CA, USA 
4  Viral Immunology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health (NIH), Bethesda, MD, USA 
5  California NanoSystems Institute, University of California, Los Angeles (UCLA), Los Angeles, CA, USA 
6  State Key Laboratory of Precision Spectroscopy, School of Physics and Electronic Science, East China Normal University (ECNU), Shanghai, China 
7  Center for Integrative Imaging, Hefei National Laboratory for Physical Sciences at the Microscale, and School of Life Sciences, University of Science and Technology of China (USTC), Hefei, Anhui, China 
Pages
1-13
Publication year
2019
Publication date
Nov 2019
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41467-019-13064-x
ProQuest document ID
2317942239
Copyright
© 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.