Abstract

Novel interventions that leverage the heterogeneity of parasite transmission are needed to achieve malaria elimination. To better understand spatial and temporal dynamics of transmission, we applied amplicon next-generation sequencing of two polymorphic gene regions (csp and ama1) to a cohort identified via reactive case detection in a high-transmission setting in western Kenya. From April 2013 to July 2014, we enrolled 442 symptomatic children with malaria, 442 matched controls, and all household members of both groups. Here, we evaluate genetic similarity between infected individuals using three indices: sharing of parasite haplotypes on binary and proportional scales and the L1 norm. Symptomatic children more commonly share haplotypes with their own household members. Furthermore, we observe robust temporal structuring of parasite genetic similarity and identify the unique molecular signature of an outbreak. These findings of both micro- and macro-scale organization of parasite populations might be harnessed to inform next-generation malaria control measures.

Details

Title
High-resolution micro-epidemiology of parasite spatial and temporal dynamics in a high malaria transmission setting in Kenya
Author
Nelson, Cody S 1   VIAFID ORCID Logo  ; Sumner, Kelsey M 2 ; Freedman, Elizabeth 3 ; Saelens, Joseph W 3 ; Obala, Andrew A 4 ; Mangeni, Judith N 5 ; Taylor, Steve M 6 ; Wendy P O’Meara 7 

 Duke Global Health Institute, Duke University, Durham, North Carolina, USA 
 Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, North Carolina, USA; Division of Infectious Diseases, Duke University School of Medicine, Durham, North Carolina, USA 
 Division of Infectious Diseases, Duke University School of Medicine, Durham, North Carolina, USA 
 School of Medicine, Moi University College of Health Sciences, Eldoret, Kenya 
 School of Nursing, Moi University College of Health Sciences, Eldoret, Kenya 
 Duke Global Health Institute, Duke University, Durham, North Carolina, USA; Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, North Carolina, USA; Division of Infectious Diseases, Duke University School of Medicine, Durham, North Carolina, USA 
 Duke Global Health Institute, Duke University, Durham, North Carolina, USA; Division of Infectious Diseases, Duke University School of Medicine, Durham, North Carolina, USA 
Pages
1-13
Publication year
2019
Publication date
Dec 2019
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2323085365
Copyright
© 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.