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Abstract
Several factors contribute to the efficiency of protein expression. Here the authors show that the identity of amino acids encoded by codons at position 3–5 significantly impact translation efficiency and protein expression levels.
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1 Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO, USA
2 Department of Structural Biology, Stanford University School of Medicine, Stanford, CA, USA; Department of Applied Physics, Stanford University, Stanford, CA, USA; Department of Genome Sciences, University of Washington, Seattle, WA, USA
3 Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO, USA; Experimental Imaging Center, IRCCS Ospedale San Raffaele, Milan, Italy
4 Department of Biology, Washington University, St Louis, MO, USA
5 Department of Bioinformatics, Institute of Biochemistry and Biophysics Polish Academy of Sciences, Warsaw, Poland
6 Department of Structural Biology, Stanford University School of Medicine, Stanford, CA, USA