Abstract

Several factors contribute to the efficiency of protein expression. Here the authors show that the identity of amino acids encoded by codons at position 3–5 significantly impact translation efficiency and protein expression levels.

Details

Title
A short translational ramp determines the efficiency of protein synthesis
Author
Verma, Manasvi 1 ; Choi, Junhong 2   VIAFID ORCID Logo  ; Cottrell, Kyle A 1 ; Lavagnino, Zeno 3 ; Thomas, Erica N 4 ; Pavlovic-Djuranovic, Slavica 1 ; Szczesny, Pawel 5   VIAFID ORCID Logo  ; Piston, David W 1   VIAFID ORCID Logo  ; Zaher, Hani S 4 ; Puglisi, Joseph D 6   VIAFID ORCID Logo  ; Djuranovic, Sergej 1   VIAFID ORCID Logo 

 Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO, USA 
 Department of Structural Biology, Stanford University School of Medicine, Stanford, CA, USA; Department of Applied Physics, Stanford University, Stanford, CA, USA; Department of Genome Sciences, University of Washington, Seattle, WA, USA 
 Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO, USA; Experimental Imaging Center, IRCCS Ospedale San Raffaele, Milan, Italy 
 Department of Biology, Washington University, St Louis, MO, USA 
 Department of Bioinformatics, Institute of Biochemistry and Biophysics Polish Academy of Sciences, Warsaw, Poland 
 Department of Structural Biology, Stanford University School of Medicine, Stanford, CA, USA 
Pages
1-15
Publication year
2019
Publication date
Dec 2019
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2328304466
Copyright
© 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.