Abstract

The dysfunction of placenta is common in somatic cell nuclear transfer (SCNT) cloned cattle and would cause aberrant fetal development and even abortion, which occurred with highest rate at the mid- to late gestation. However, the mechanism of abnormal placentas was unclear. To analyze the transcriptome-wide characteristics of abnormal placentas in SCNT cloned cattle, the mRNA, lncRNA and miRNA of placental cotyledon tissue at day 180 after gestation were sequenced. A total of 19,055 mRNAs, 30,141 lncRNAs and 684 miRNAs were identified. Compared with control group, 362 mRNAs, 1,272 lncRNAs and nine miRNAs (six known and three novel miRNAs) were differentially expressed (fold change ≥ 2 and P-value < 0.05). The differentially expressed genes were functionally enriched in urea and ions transmembrane transport, which indicated that the maternal-fetal interactions were disturbed in impaired placentas. Furthermore, the competing endogenous RNAs (ceRNAs) networks were identified to illustrate their roles in abnormal placental morphology. The present research would be helpful to discover the mechanism of late gestational abnormality of SCNT cattle by provides important genomic information and insights.

Details

Title
Transcriptome-wide analysis of the SCNT bovine abnormal placenta during mid- to late gestation
Author
Gao, Guangqi 1 ; Wang, Shenyuan 2 ; Zhang, Jiaqi 3 ; Su, Guanghua 4 ; Zheng, Zhong 4 ; Bai, Chunling 4 ; Yang, Lei 4   VIAFID ORCID Logo  ; Zhuying Wei 4 ; Wang, Xiuying 5 ; Liu, Xiao 2 ; Guo, Ziru 2 ; Li, Guangpeng 4   VIAFID ORCID Logo  ; Su, Xiaohu 6   VIAFID ORCID Logo  ; Zhang, Li 1 

1  The State key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, China; College of Life Science, Inner Mongolia University, Hohhot, China; College of Life Science, Inner Mongolia Agricultural University, Hohhot, China 
2  College of Life Science, Inner Mongolia Agricultural University, Hohhot, China 
3  State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China 
4  The State key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, China; College of Life Science, Inner Mongolia University, Hohhot, China 
5  Inner Mongolia Radio and TV University, Hohhot, China 
6  The State key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, China; Key Laboratory of Gene Engineering of the Ministry of Education, Guangzhou Key Laboratory of Healthy Aging Research and State Key Laboratory of Biocontrol, SYSU-BCM JointResearch Center, School of Life Sciences, Sun Yat-sen University, Guangzhou, China 
Pages
1-10
Publication year
2019
Publication date
Dec 2019
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-019-56566-w
ProQuest document ID
2330969917
Copyright
© 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.