Abstract

In many plant species, roots maintain specific growth angles relative to the direction of gravity, known as gravitropic set point angles (GSAs). These contribute to the efficient acquisition of water and nutrients. AtLAZY1/LAZY1-LIKE (LZY) genes are involved in GSA control by regulating auxin flow toward the direction of gravity in Arabidopsis. Here, we demonstrate that RCC1-like domain (RLD) proteins, identified as LZY interactors, are essential regulators of polar auxin transport. We show that interaction of the CCL domain of LZY with the BRX domain of RLD is important for the recruitment of RLD from the cytoplasm to the plasma membrane by LZY. A structural analysis reveals the mode of the interaction as an intermolecular β-sheet in addition to the structure of the BRX domain. Our results offer a molecular framework in which gravity signal first emerges as polarized LZY3 localization in gravity-sensing cells, followed by polar RLD1 localization and PIN3 relocalization to modulate auxin flow.

Details

Title
Polar recruitment of RLD by LAZY1-like protein during gravity signaling in root branch angle control
Author
Furutani, Masahiko 1   VIAFID ORCID Logo  ; Hirano, Yoshinori 2   VIAFID ORCID Logo  ; Nishimura, Takeshi 3 ; Nakamura, Moritaka 3 ; Taniguchi, Masatoshi 4   VIAFID ORCID Logo  ; Suzuki, Kanako 4 ; Oshida, Ryuichiro 4 ; Kondo, Chiemi 4 ; Sun, Song 5 ; Kato, Kagayaki 6 ; Fukao, Yoichiro 7 ; Hakoshima, Toshio 8 ; Miyo Terao Morita 3   VIAFID ORCID Logo 

1  College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China; FAFU-UCR Joint Center, Fujian Provincial Key Laboratory of Haixia Applied Plant System Biology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China; Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, Japan 
2  Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Japan; Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan 
3  Division of Plant Environmental Responses, National Institute for Basic Biology, Myodaiji, Okazaki, Japan 
4  Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, Japan 
5  College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China; FAFU-UCR Joint Center, Fujian Provincial Key Laboratory of Haixia Applied Plant System Biology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China 
6  Laboratory of Biological Diversity, National Institute for Basic Biology, Myodaiji, Okazaki, Japan; Bioimage Informatics Group, Exploratory Research Center on Life and Living Systems (ExCELLS), National Institute of Natural Sciences, Myodaiji, Okazaki, Japan 
7  Department of Bioinformatics, Ritsumeikan University, Kusatsu, Japan 
8  Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Japan 
Pages
1-13
Publication year
2020
Publication date
2020
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41467-019-13729-7
ProQuest document ID
2332339529
Copyright
© 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.