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© 2019. This work is licensed under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

In terms of pest control, RNAi-mediated silencing has shown excellent efficacy and has been applied in the control of a number of coleopteran insects, including the Western corn rootworm Diabrotica virgifera virgifera [19], red flour beetle Tribolium castaneum [20], and the Colorado potato beetle Leptinotarsa decemlineata [20,21]. [...]our preliminary experimental results indicated that dietary RNAi targeting multiple genes is particularly effective against H. vigintioctopunctata, indicating that RNAi has potential utility in the control of this species. [...]we reasoned that a molecular investigation of H. vigintioctopunctata reproduction would provide important insights with regard to identifying novel target sites for population control. [...]we performed comparative transcriptome analysis to identify the genes that were differentially expressed between ovaries and testes, among which, 13 and 16 candidate unigenes that were upregulated in the ovary and testis, respectively, were selected to confirm their altered expression levels via RT-qPCR and semi-RT-qPCR analyses. [...]we investigated the effects of silencing two ovary-specific genes, RC2-like and PSHS-like, on the mortality, percentage infertility, pre-oviposition period, and fecundity of adult females, along with the number of eggs laid daily and egg hatching rate.

Details

Title
De Novo Transcriptome Analysis Reveals Abundant Gonad-specific Genes in the Ovary and Testis of Henosepilachna vigintioctopunctata
Author
Guo, Wei; Lü, Jing; Guo, Mujuan; Chen, Shimin; Qiu, Baoli; Wen Sang; Yang, Chunxiao; Zhang, Youjun; Pan, Huipeng
Publication year
2019
Publication date
2019
Publisher
MDPI AG
ISSN
16616596
e-ISSN
14220067
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2332368119
Copyright
© 2019. This work is licensed under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.