Abstract

Molecular imaging of atherosclerosis by Magnetic Resonance Imaging (MRI) has been impaired by a lack of validation of the specific substrate responsible for the molecular imaging signal. We therefore aimed to investigate the additive value of mass spectrometry imaging (MSI) of atherosclerosis-affine Gadofluorine P for molecular MRI of atherosclerotic plaques. Atherosclerotic Ldlr−/− mice were investigated by high-field MRI (7 T) at different time points following injection of atherosclerosis-affine Gadofluorine P as well as at different stages of atherosclerosis formation (4, 8, 16 and 20 weeks of HFD). At each imaging time point mice were immediately sacrificed after imaging and aortas were excised for mass spectrometry imaging: Matrix Assisted Laser Desorption Ionization (MALDI) Imaging and Laser Ablation – Inductively Coupled Plasma – Mass Spectrometry (LA-ICP-MS) imaging. Mass spectrometry imaging allowed to visualize the localization and measure the concentration of the MR imaging probe Gadofluorine P in plaque tissue ex vivo with high spatial resolution and thus adds novel and more target specific information to molecular MR imaging of atherosclerosis.

Details

Title
Mass Spectrometry Imaging of atherosclerosis-affine Gadofluorine following Magnetic Resonance Imaging
Author
Lohöfer Fabian 1 ; Buchholz, Rebecca 2 ; Glinzer Almut 1 ; Huber, Katharina 3 ; Haas, Helena 1 ; Kaissis Georgios 1 ; Feuchtinger Annette 3 ; Aichler Michaela 3 ; Sporns, Peter B 4 ; Höltke Carsten 4 ; Stölting Miriam 4 ; Schilling, Franz 5 ; Botnar, René M 6   VIAFID ORCID Logo  ; Kimm, Melanie A 1   VIAFID ORCID Logo  ; Faber, Cornelius 7   VIAFID ORCID Logo  ; Walch, Axel K 3 ; Zernecke Alma 8 ; Karst Uwe 9   VIAFID ORCID Logo  ; Wildgruber Moritz 10 

1  Technical University of Munich, Department of Diagnostic and Interventional Radiology, School of Medicine & Klinikum rechts der Isar, Munich, Germany (GRID:grid.6936.a) (ISNI:0000000123222966) 
2  Westfälische Wilhelms-Universität, Department for Analytical Chemistry, Münster, Germany (GRID:grid.5949.1) (ISNI:0000 0001 2172 9288) 
3  Helmholtz Zentrum München, Research Unit Analytical Pathology, German Research Center for Environmental Health, Neuherberg, Germany (GRID:grid.4567.0) (ISNI:0000 0004 0483 2525) 
4  Westfälische Wilhelms-Universität, Translational Research Imaging Center, Department of Clinical Radiology, Münster, Germany (GRID:grid.5949.1) (ISNI:0000 0001 2172 9288) 
5  Technical University of Munich, Department of Nuclear Medicine, School of Medicine & Klinikum rechts der Isar, Munich, Germany (GRID:grid.6936.a) (ISNI:0000000123222966) 
6  King’s College London, School of Biomedical Engineering and Imaging Sciences, London, UK (GRID:grid.13097.3c) (ISNI:0000 0001 2322 6764) 
7  Westfälische Wilhelms-Universität, Translational Research Imaging Center, Department of Clinical Radiology, Münster, Germany (GRID:grid.5949.1) (ISNI:0000 0001 2172 9288) ; DFG Cluster of Excellence EXC 1003 ‘Cells in Motion’, Münster, Germany (GRID:grid.5949.1) 
8  Universitätsklinikum Würzburg, Institute for Experimental Biomedicine, Würzburg, Germany (GRID:grid.411760.5) (ISNI:0000 0001 1378 7891) 
9  Westfälische Wilhelms-Universität, Department for Analytical Chemistry, Münster, Germany (GRID:grid.5949.1) (ISNI:0000 0001 2172 9288) ; DFG Cluster of Excellence EXC 1003 ‘Cells in Motion’, Münster, Germany (GRID:grid.5949.1) 
10  Technical University of Munich, Department of Diagnostic and Interventional Radiology, School of Medicine & Klinikum rechts der Isar, Munich, Germany (GRID:grid.6936.a) (ISNI:0000000123222966) ; Westfälische Wilhelms-Universität, Translational Research Imaging Center, Department of Clinical Radiology, Münster, Germany (GRID:grid.5949.1) (ISNI:0000 0001 2172 9288) ; DFG Cluster of Excellence EXC 1003 ‘Cells in Motion’, Münster, Germany (GRID:grid.5949.1) 
Publication year
2020
Publication date
2020
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-019-57075-6
ProQuest document ID
2342987305
Copyright
This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.