Abstract

The epidermal growth factor receptor (EGFR) inhibitors such as erlotinib and gefitinib are widely used for treatment of non-small cell lung cancer (NSCLC), but they have shown limited efficacy in an unselected population of patients. The KRAS mutations, which are identified in approximately 20% of NSCLC patients, have shown to be associated with the resistance to the EGFR tyrosine kinase inhibitors (TKIs). Currently, there is no clinically available targeted therapy which can effectively inhibit NSCLC tumors harboring KRAS mutations. This study aims to show the effectiveness of KYA1797K, a small molecule which revealed anti-cancer effect in colorectal cancer by destabilizing Ras via inhibiting the Wnt/β-catenin pathway, for the treatment of KRAS-mutated NSCLC. While erlotinib fail to have anti-transforming effect in NSCLC cell lines harboring KRAS mutations, KYA1797K effectively inhibited the Ras-ERK pathway in KRAS-mutant NSCLC cell lines. As a result, KYA1797K treatment suppressed the growth and transformation of KRAS mutant NSCLC cells and also induced apoptosis. Furthermore, KYA1797K effectively inhibited Kras-driven tumorigenesis in the KrasLA2 mouse model by suppressing the Ras-ERK pathway. The destabilization of Ras via inhibition of the Wnt/β-catenin pathway is a potential therapeutic strategy for KRAS-mutated NSCLC that is resistant to EGFR TKI.

Details

Title
A Ras destabilizer KYA1797K overcomes the resistance of EGFR tyrosine kinase inhibitor in KRAS-mutated non-small cell lung cancer
Author
Park, Jieun 1 ; Yong-Hee, Cho 1 ; Wook-Jin, Shin 1 ; Sang-Kyu, Lee 1 ; Lee, JaeHeon 1 ; Kim, Taehyung 1 ; Pu-Hyeon, Cha 1 ; Yang Jee Sun 1 ; Cho Jaebeom 2 ; Min Do Sik 3 ; Han Gyoonhee 1 ; Ho-Young, Lee 2 ; Kang-Yell, Choi 1 

 Yonsei University, Translational Research Center for Protein Function Control, Seoul, Korea (GRID:grid.15444.30) (ISNI:0000 0004 0470 5454); Yonsei University, Department of Biotechnology, College of Life Science and Biotechnology, Seoul, Korea (GRID:grid.15444.30) (ISNI:0000 0004 0470 5454) 
 Seoul National University, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul, Korea (GRID:grid.31501.36) (ISNI:0000 0004 0470 5905) 
 Yonsei University, Translational Research Center for Protein Function Control, Seoul, Korea (GRID:grid.15444.30) (ISNI:0000 0004 0470 5454); Pusan National University, Department of Molecular Biology, College of Natural Science, Pusan, Korea (GRID:grid.262229.f) (ISNI:0000 0001 0719 8572) 
Publication year
2019
Publication date
Jan 2019
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2344211384
Copyright
This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.