Abstract

Canonical roles for macrophages in mediating the fibrotic response after a heart attack include extracellular matrix turnover and activation of cardiac fibroblasts to initiate collagen deposition. Here we reveal that macrophages directly contribute collagen to the forming post-injury scar. Unbiased transcriptomics shows an upregulation of collagens in both zebrafish and mouse macrophages following heart injury. Adoptive transfer of macrophages, from either collagen-tagged zebrafish or adult mouse GFPtpz-collagen donors, enhances scar formation via cell autonomous production of collagen. In zebrafish, the majority of tagged collagen localises proximal to the injury, within the overlying epicardial region, suggesting a possible distinction between macrophage-deposited collagen and that predominantly laid-down by myofibroblasts. Macrophage-specific targeting of col4a3bpa and cognate col4a1 in zebrafish significantly reduces scarring in cryoinjured hosts. Our findings contrast with the current model of scarring, whereby collagen deposition is exclusively attributed to myofibroblasts, and implicate macrophages as direct contributors to fibrosis during heart repair.

Macrophages mediate the fibrotic response after a heart attack by extracellular matrix turnover and cardiac fibroblasts activation. Here the authors identify an evolutionarily-conserved function of macrophages that contributes directly to the forming post-injury scar through cell-autonomous deposition of collagen.

Details

Title
Macrophages directly contribute collagen to scar formation during zebrafish heart regeneration and mouse heart repair
Author
Simões, Filipa C 1   VIAFID ORCID Logo  ; Cahill, Thomas J 2 ; Kenyon, Amy 3 ; Gavriouchkina Daria 4   VIAFID ORCID Logo  ; Vieira, Joaquim M 5   VIAFID ORCID Logo  ; Sun, Xin 5 ; Pezzolla Daniela 6 ; Ravaud Christophe 5   VIAFID ORCID Logo  ; Masmanian Eva 3 ; Weinberger, Michael 7 ; Mayes, Sarah 7 ; Lemieux, Madeleine E 8   VIAFID ORCID Logo  ; Barnette, Damien N 9 ; Gunadasa-Rohling Mala 9 ; Williams, Ruth M 3 ; Greaves, David R 10   VIAFID ORCID Logo  ; Trinh, Le A 11 ; Fraser, Scott E 11 ; Dallas, Sarah L 12 ; Choudhury, Robin P 6   VIAFID ORCID Logo  ; Sauka-Spengler Tatjana 3   VIAFID ORCID Logo  ; Riley, Paul R 5   VIAFID ORCID Logo 

 University of Oxford, Department of Physiology, Anatomy and Genetics, Oxford, UK (GRID:grid.4991.5) (ISNI:0000 0004 1936 8948); University of Oxford, Radcliffe Department of Medicine, MRC Weatherall Institute of Molecular Medicine, Oxford, UK (GRID:grid.4991.5) (ISNI:0000 0004 1936 8948); University of Oxford, BHF Oxbridge Centre of Regenerative Medicine, Oxford, UK (GRID:grid.4991.5) (ISNI:0000 0004 1936 8948) 
 University of Oxford, Department of Physiology, Anatomy and Genetics, Oxford, UK (GRID:grid.4991.5) (ISNI:0000 0004 1936 8948); University of Oxford, BHF Oxbridge Centre of Regenerative Medicine, Oxford, UK (GRID:grid.4991.5) (ISNI:0000 0004 1936 8948); University of Oxford, Division of Cardiovascular Medicine, Radcliffe Department of Medicine, Oxford, UK (GRID:grid.4991.5) (ISNI:0000 0004 1936 8948) 
 University of Oxford, Radcliffe Department of Medicine, MRC Weatherall Institute of Molecular Medicine, Oxford, UK (GRID:grid.4991.5) (ISNI:0000 0004 1936 8948) 
 University of Oxford, Radcliffe Department of Medicine, MRC Weatherall Institute of Molecular Medicine, Oxford, UK (GRID:grid.4991.5) (ISNI:0000 0004 1936 8948); Molecular Genetics Unit, Okinawa Institute of Science & Technology, Onna, Japan (GRID:grid.250464.1) (ISNI:0000 0000 9805 2626) 
 University of Oxford, Department of Physiology, Anatomy and Genetics, Oxford, UK (GRID:grid.4991.5) (ISNI:0000 0004 1936 8948); University of Oxford, BHF Oxbridge Centre of Regenerative Medicine, Oxford, UK (GRID:grid.4991.5) (ISNI:0000 0004 1936 8948) 
 University of Oxford, Division of Cardiovascular Medicine, Radcliffe Department of Medicine, Oxford, UK (GRID:grid.4991.5) (ISNI:0000 0004 1936 8948) 
 University of Oxford, Department of Physiology, Anatomy and Genetics, Oxford, UK (GRID:grid.4991.5) (ISNI:0000 0004 1936 8948); University of Oxford, Radcliffe Department of Medicine, MRC Weatherall Institute of Molecular Medicine, Oxford, UK (GRID:grid.4991.5) (ISNI:0000 0004 1936 8948) 
 Bioinfo, Plantagenet, Canada (GRID:grid.4991.5) 
 University of Oxford, Department of Physiology, Anatomy and Genetics, Oxford, UK (GRID:grid.4991.5) (ISNI:0000 0004 1936 8948) 
10  University of Oxford, Sir William Dunn School of Pathology, Oxford, UK (GRID:grid.4991.5) (ISNI:0000 0004 1936 8948) 
11  University of Southern California, Translational Imaging Centre, Los Angeles, USA (GRID:grid.42505.36) (ISNI:0000 0001 2156 6853) 
12  School of Dentistry, University of Missouri-Kansas City, Kansas City, USA (GRID:grid.266756.6) (ISNI:0000 0001 2179 926X) 
Publication year
2020
Publication date
2020
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2348775942
Copyright
This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.