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© 2019. This work is published under https://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Background: Atypical pneumonia is an upper and lower respiratory tract infection. Mycoplasma pneumoniae is a major cause of community-acquired pneumonia (CAP).

Objectives: The present study aimed to determine the prevalence of atypical pneumonia caused by M. pneumonia by culture and molecular PCR methods in Tehran.

Methods: In the present study, 102 samples of throat swab were collected from patients with respiratory infections. All samples were cultured in liquid PPLO Broth And solid PPLO agar media (1% glucose and 20% horse serum). The PCR technique with specific primers was implemented after culture and genome extraction through phenol-chloroform technique.

Results: In this study, 27 (26.47%) colonies of Mycoplasma were isolated on PPLO agar medium. Using specific primers, it was found that 33 samples (32.4%) were positive in terms of Mycoplasma genus and 14 samples (13.7%) were positive for the presence of M. pneumonia.

Conclusions: Mycoplasma pneumonia is a pathogen that causes respiratory tract infections in humans. Molecular PCR method is aquick and sensitive technique that has higher sensitivity and specificity than other methods. The obtained results may contribute to the specific treatment of some patients with symptoms of respiratory infections.

Details

Title
The Prevalence of Atypical Pneumonia Caused by Mycoplasma pneumoniae (P1 gene) in Patients with Respiratory Infections by Culture and Molecular PCR Methods in Tehran, Iran
Author
Babaei, Zahra 1 ; Pouladi, Iman 1 ; Ashtari, Abbass 2 ; Azimi, Gasem 3 ; Niakan, Mohammad 1 

 Department of Medical Microbiology, Faculty of Medicine, Shahed University of Medical Sciences, Tehran, Iran 
 Mycoplasma Reference Laboratory, Razi Vaccine and Serum Research Institute, Karaj, Iran 
 Department of Internal Medicine, Shahed University of Medical Sciences, Tehran, Iran 
Pages
1-6
Section
Research Article
Publication year
2019
Publication date
Nov 2019
Publisher
Ahvaz Jundishapur University of Medical Sciences
ISSN
20083645
e-ISSN
20084161
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2350105830
Copyright
© 2019. This work is published under https://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.