Abstract

Signaling lymphocytic activation molecule family member 8 (SLAMF8) / B-lymphocyte activator macrophage expressed/CD353 is a member of the CD2 family. SLAMF8 suppresses macrophage function but enhances the growth of neoplastic mast cells via SHP-2. In this study, we found that some anaplastic large cell lymphoma (ALCL) samples were immunohistochemically positive for SLAMF8. However, we found no significant differences between SLAMF8-positive and SLAMF8-negative ALCL samples with respect to age, gender, site, or prognosis. We also identified SLAMF8 expression in ALCL cell lines, Karpas299, and SU-DHL-1. SLAMF8 knockdown decreased the activation of SHP-2 and the growth of these cell lines, and increased the apoptosis of these cell lines. In addition, we observed the interaction between SLAMF8 and SHP-2 in these cell lines using the DuoLink in situ kit. Taken together, these results suggest that SLAMF8 may enhance the growth of ALCL via SHP-2 interaction.

Details

Title
SLAM family member 8 is expressed in and enhances the growth of anaplastic large cell lymphoma
Author
Sugimoto Akihiko 1   VIAFID ORCID Logo  ; Kataoka, Tatsuki R 1 ; Ito Hiroaki 1 ; Kitamura Kyohei 1 ; Saito Narumi 1 ; Hirata Masahiro 1 ; Ueshima Chiyuki 1 ; Takei Yusuke 2 ; Moriyoshi Koki 3 ; Otsuka Yasuyuki 4 ; Nishikori Momoko 4 ; Takaori-Kondo Akifumi 4   VIAFID ORCID Logo  ; Haga Hironori 1 

1  Kyoto University Hospital, Department of Diagnostic Pathology, Kyoto, Japan (GRID:grid.411217.0) (ISNI:0000 0004 0531 2775) 
2  Kyoto University Hospital, Department of Diagnostic Pathology, Kyoto, Japan (GRID:grid.411217.0) (ISNI:0000 0004 0531 2775); Saiseikai-Noe Hospital, Department of Diagnostic Pathology, Osaka, Japan (GRID:grid.411217.0) 
3  Kyoto University Hospital, Department of Diagnostic Pathology, Kyoto, Japan (GRID:grid.411217.0) (ISNI:0000 0004 0531 2775); Kyoto Medical Center, Department of Diagnostic Pathology, Kyoto, Japan (GRID:grid.410835.b) 
4  Kyoto University Hospital, Department of Hematology/Oncology, Kyoto, Japan (GRID:grid.411217.0) (ISNI:0000 0004 0531 2775) 
Publication year
2020
Publication date
2020
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-020-59530-1
ProQuest document ID
2354721078
Copyright
This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.