Indium-111-labeled CD166-targeted peptide as a potential nuclear imaging agent for detecting colorectal cancer stem-like cells in a xenograft mouse model

Abstract

Background

Cancer stem cells (CSCs) are involved in drug resistance, metastasis, and relapse of cancers, which can significantly affect tumor therapy. Hence, to develop specifically therapeutic target probe at CSCs for improvement of survival and quality of life of cancer patients is urgently needed. The CD166 protein has been suggested to be involved in colorectal cancer (CRC) tumorigenesis and to be considered a marker for colorectal CSCs (CRCSCs) detection. In this study, therefore, we attend to apply a nuclear imaging agent probe, Glycine₁₈-Cystine-linked CD166-targeted peptides (CD166tp-G₁₈C), to detect the changes of CD166 level in a CRC xenograft mouse model.

Results

We isolated the CD166-positive cells from the HCT15 CRC cell line (CD166⁺HCT15) and evaluated their morphology and ability of clone formation, migration, protein expression, and drug resistance. The CD166-positive HCT15 cells display the CSCs characteristics. We discovered and designed a CD166-targeted peptide (CD166tp-G₁₈C) as a targeted probe of CRC stem-like cell for cell binding assay. The CD166tp-G₁₈C confirmed the CD166 protein targeting ability in CD166⁺HCT15 cells. The diethylenetriaminopentaacetic acid (DTPA)-conjugated CD166tp-G₁₈C further was labeled with indium-111 (¹¹¹In-DTPA-CD166tp-G₁₈C) as nuclear imaging agent for imaging and bio-distribution analysis in vivo. Finally, we observed that the ¹¹¹In-DTPA-CD166tp-G₁₈C was significantly enhanced in tumor tissues of CD166⁺HCT15 xenograft mice as compared to the non-CD166tp-G₁₈C control.

Conclusions

Our results indicated that the indium-111-labeled CD166tp-G₁₈C may be served as a powerful tool for colorectal CSCs nuclear imaging in the CRC patients.