Global transcriptome analysis of pig induced pluripotent stem cells derived from six and four reprogramming factors

Abstract

Pigs are important, both for agriculture and as animal models for human diseases. However, due to the lack of embryonic stem cells, the possibility of genetic modification is quite limited. To overcome this limitation, induced pluripotent stem (iPS) cells have been derived from pigs. Despite the public availability of a large number of expression datasets from mice, rats, and primates-derived iPS cells, the expression profile of pig-derived iPS cells is quite limited. Furthermore, there is no dataset focused on the profiling of pig-derived iPS cell with six reprogramming factors (Oct3/4, Sox2, Klf4, c-Myc, Lin28, and Nanog). Here, we used Illumina RNA sequencing platform to characterize the mRNA expression of four-factor derived and six-factor derived pig iPS cells. We observed that the expression levels of whole genes in our established six factors derived iPS cells and parent fibroblast, and compared with that of iPS cells with four factors in public database. These data are valuable in understanding species difference in the reprogramming process of stem cells, and could help identify the key regulating genes involved in the process.

Design Type(s)	transcription profiling identification objective • cell type comparison design
Measurement Type(s)	transcriptional profiling assay
Technology Type(s)	RNA sequencing
Factor Type(s)	pluripotency induction technique • technical replicate • biological replicate
Sample Characteristic(s)	Sus scrofa • induced pluripotent stem cell line cell • embryonic fibroblast

Machine-accessible metadata file describing the reported data (ISA-Tab format)