Abstract

In humans, parthenogenesis and androgenesis occur naturally in mature cystic ovarian teratomas and androgenetic complete hydatidiform moles (CHM), respectively. Our previous study has reported human parthenogenetic induced pluripotent stem cells from ovarian teratoma–derived fibroblasts and screening of imprinted genes using genome-wide DNA methylation analysis. However, due to the lack of the counterparts of uniparental cells, identification of new imprinted differentially methylated regions has been limited. CHM are inherited from only the paternal genome. In this study, we generated human androgenetic induced pluripotent stem cells (AgHiPSCs) from primary androgenetic fibroblasts derived from CHM. To investigate the pluripotency state of AgHiPSCs, we analyzed their cellular and molecular characteristics. We tested the DNA methylation status of imprinted genes using bisulfite sequencing and demonstrated the androgenetic identity of AgHiPSCs. AgHiPSCs might be an attractive alternative source of human androgenetic embryonic stem cells. Furthermore, AgHiPSCs can be used in regenerative medicine, for analysis of genomic imprinting, to study imprinting-related development, and for disease modeling in humans.

Details

Title
Generation of human androgenetic induced pluripotent stem cells
Author
Choi Na Young 1 ; Bang, Jin Seok 1 ; Park Yo Seph 2 ; Lee, Minseong 1 ; Hwang, Han Sung 3 ; Ko Kisung 4 ; Myung Soon Chul 5 ; Tapia, Natalia 6   VIAFID ORCID Logo  ; Schöler, Hans R 7   VIAFID ORCID Logo  ; Kim, Gwang Jun 8 ; Ko Kinarm 9 

1  Konkuk University, Department of Stem Cell Biology, School of Medicine, Seoul, Republic of Korea (GRID:grid.258676.8) (ISNI:0000 0004 0532 8339); Konkuk University, Center for Stem Cell Research, Institute of Advanced Biomedical Science, Seoul, Republic of Korea (GRID:grid.258676.8) (ISNI:0000 0004 0532 8339) 
2  TJC Life Research and Development Center, TJC Life, Department of Stem Cell Research, Seoul, Republic of Korea (GRID:grid.258676.8) 
3  Konkuk University School of Medicine, Department of Obstetrics and Gynecology, Research Institute of Medical Science, Seoul, Republic of Korea (GRID:grid.258676.8) (ISNI:0000 0004 0532 8339) 
4  Chung-Ang University, Department of Medicine, College of Medicine, Seoul, Republic of Korea (GRID:grid.254224.7) (ISNI:0000 0001 0789 9563) 
5  Chung-Ang University College of Medicine, Department of Urology, Seoul, Republic of Korea (GRID:grid.254224.7) (ISNI:0000 0001 0789 9563) 
6  Spanish National Research Council, Institute of Biomedicine of Valencia, Valencia, Spain (GRID:grid.4711.3) (ISNI:0000 0001 2183 4846) 
7  Max Planck Institute for Molecular Biomedicine, Department of Cell and Developmental Biology, Münster, Germany (GRID:grid.461801.a) (ISNI:0000 0004 0491 9305); Medical Faculty, University of Münster, Münster, Germany (GRID:grid.5949.1) (ISNI:0000 0001 2172 9288) 
8  Chung-Ang University Hospital, Chung-Ang University College of Medicine, Department of Obstetrics and Gynecology, Seoul, Republic of Korea (GRID:grid.5949.1) 
9  Konkuk University, Department of Stem Cell Biology, School of Medicine, Seoul, Republic of Korea (GRID:grid.258676.8) (ISNI:0000 0004 0532 8339); Konkuk University, Center for Stem Cell Research, Institute of Advanced Biomedical Science, Seoul, Republic of Korea (GRID:grid.258676.8) (ISNI:0000 0004 0532 8339); Konkuk University, Research Institute of Medical Science, Seoul, Republic of Korea (GRID:grid.258676.8) (ISNI:0000 0004 0532 8339) 
Publication year
2020
Publication date
2020
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-020-60363-1
ProQuest document ID
2366598909
Copyright
This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.