Abstract

Porphyra 334 (P334), a mycosporine-like amino acid (MAA), is a secondary metabolite found in diverse marine and terrestrial organisms and has several beneficial effects on fibroblast proliferation, wound healing, and antioxidant activity. Here, we report that P334 accelerates the cell reprogramming process of mouse tail-tip fibroblasts (TTFs) and human dermal papilla (HDP) cells into induced pluripotent stem cells (iPSCs). We found that P334 significantly improved the cell reprogramming efficiency by activating the tri-methylation of histone 3 lysine 4 (H3K4me3), which controls mesenchymal to epithelial transition (MET) during the reprogramming process. Thus, we found that P334 directly regulates epigenetic changes, providing an efficient approach for natural compound-based cell reprogramming.

Details

Title
Acceleration of somatic cell reprogramming into the induced pluripotent stem cell using a mycosporine-like amino acid, Porphyra 334
Author
Yoo Junsang 1 ; Kim Junyeop 2 ; Lee, Jeong Hun 3 ; Kim Hyein 3 ; Jang, Sung Joo 3 ; Seo, Hyo Hyun 3 ; Oh, Seung Taek 3 ; Hyeon, Seung Jae 4 ; Ryu Hoon 4 ; Kim, Jongpil 2 ; Moh Sang Hyun 3   VIAFID ORCID Logo 

1  Anti-aging Research Institute, BIO-FD&C Co., Ltd, Inchon, Korea; Center for Neuroscience, Brain Science Institute, Korea Institute of Science and Technology, Seoul, Korea (GRID:grid.35541.36) (ISNI:0000000121053345) 
2  Dongguk University, Department of Biomedical Engineering, Seoul, Korea (GRID:grid.255168.d) (ISNI:0000 0001 0671 5021) 
3  Anti-aging Research Institute, BIO-FD&C Co., Ltd, Inchon, Korea (GRID:grid.255168.d) 
4  Center for Neuroscience, Brain Science Institute, Korea Institute of Science and Technology, Seoul, Korea (GRID:grid.35541.36) (ISNI:0000000121053345) 
Publication year
2020
Publication date
2020
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-020-60680-5
ProQuest document ID
2367848812
Copyright
This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.