It appears you don't have support to open PDFs in this web browser. To view this file, Open with your PDF reader
Abstract
Directed breeding of horticultural crops is essential for increasing yield, nutritional content, and consumer-valued characteristics such as shape and color of the produce. However, limited genetic diversity restricts the amount of crop improvement that can be achieved through conventional breeding approaches. Natural genetic changes in cis-regulatory regions of genes play important roles in shaping phenotypic diversity by altering their expression. Utilization of CRISPR/Cas editing in crop species can accelerate crop improvement through the introduction of genetic variation in a targeted manner. The advent of CRISPR/Cas-mediated cis-regulatory region engineering (cis-engineering) provides a more refined method for modulating gene expression and creating phenotypic diversity to benefit crop improvement. Here, we focus on the current applications of CRISPR/Cas-mediated cis-engineering in horticultural crops. We describe strategies and limitations for its use in crop improvement, including de novo cis-regulatory element (CRE) discovery, precise genome editing, and transgene-free genome editing. In addition, we discuss the challenges and prospects regarding current technologies and achievements. CRISPR/Cas-mediated cis-engineering is a critical tool for generating horticultural crops that are better able to adapt to climate change and providing food for an increasing world population.
You have requested "on-the-fly" machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Show full disclaimer
Neither ProQuest nor its licensors make any representations or warranties with respect to the translations. The translations are automatically generated "AS IS" and "AS AVAILABLE" and are not retained in our systems. PROQUEST AND ITS LICENSORS SPECIFICALLY DISCLAIM ANY AND ALL EXPRESS OR IMPLIED WARRANTIES, INCLUDING WITHOUT LIMITATION, ANY WARRANTIES FOR AVAILABILITY, ACCURACY, TIMELINESS, COMPLETENESS, NON-INFRINGMENT, MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. Your use of the translations is subject to all use restrictions contained in your Electronic Products License Agreement and by using the translation functionality you agree to forgo any and all claims against ProQuest or its licensors for your use of the translation functionality and any output derived there from. Hide full disclaimer
Details
1 Shandong Agricultural University, College of Horticultural Science and Engineering, Tai’an, China (GRID:grid.440622.6) (ISNI:0000 0000 9482 4676); University of Georgia, Center for Applied Genetic Technologies, Athens, USA (GRID:grid.213876.9) (ISNI:0000 0004 1936 738X)
2 University of Georgia, Institute for Plant Breeding, Genetics and Genomics, Athens, USA (GRID:grid.213876.9) (ISNI:0000 0004 1936 738X)
3 University of Georgia, Center for Applied Genetic Technologies, Athens, USA (GRID:grid.213876.9) (ISNI:0000 0004 1936 738X); University of Georgia, Institute for Plant Breeding, Genetics and Genomics, Athens, USA (GRID:grid.213876.9) (ISNI:0000 0004 1936 738X); University of Georgia, Department of Horticulture, Athens, USA (GRID:grid.213876.9) (ISNI:0000 0004 1936 738X)