Dlgap1 negatively regulates browning of white fat cells through effects on cell proliferation and apoptosis

Abstract

Background

Obesity is a metabolic imbalance characterized by excessive deposition of white fat. The browning of white fat can effectively treat obesity and related diseases. Although Dlgap1 (Discs, Large (Drosophila) Homolog-Associated Protein 1) is suspected to have an effect on this process, no empirical evidence is available.

Methods

To understand the role of Dlgap1, we cultured white and brown fat cells, then performed overexpression and knockout experiments.

Results

We found that Dlgap1 overexpression in brown adipocytes inhibits brown-fat-related gene expression, promotes white-fat-related genes, while also increasing brown-adipocyte proliferation and apoptosis. However, the gene overexpression has no effect on brown adipocyte maturation. Knocking out Dlgap1 in white fat cells promotes the expression and inhibition of brown-fat-related and white-fat-related genes, respectively. Additionally, the knockout inhibits white fat cell proliferation and apoptosis, while also promoting their maturation.

Conclusions

Dlgap1 negatively regulates the browning of white adipocytes by influencing cell proliferation and apoptosis.