Abstract

Objective(s): In this study, a cocktail of probe drugs was used to assess whether lentinan could influence the activities of rat enzymes CYP3A4, CYP2D6, CYP1A2, CYP2C19, and CYP2C9 in vivo.

Materials and Methods: Fourteen days after intraperitoneal injection of lentinan, rats were given an oral dose of a cocktail solution containing phenacetin, tolbutamide, omeprazole, metoprolol, and midazolam. Then, we obtained blood in specific durations for the determination of plasma concentration of the probe drugs using UPLC-MS/MS. We also evaluated the pharmacokinetic parameters using the DAS 2.0 software.

Results: We found that various concentrations of lentinan increased the activity of rat CYP1A2, CYP3A4, CYP2D6, and CYP2C19 but not CYP2C9.

Conclusion: These findings suggest that clinical application of lentinan combination with CYP3A4, CYP1A2, CYP2C19, or CYP2D6 should be given careful consideration as this may lead to herb-drug interactions and hence treatment failure.

Details

Title
Evaluation of lentinan effects on cytochrome P450 activity in rats by a cocktail method
Author
Lin, Yiping  VIAFID ORCID Logo  ; Wei, Yanli; Hu, Xiaoxia; Wu, Mei-Ling; Yao, Jingchan; Ying, Xiaoqian; Fu, Xiaoyan; Ding, Mingxing; Liman Qiao  VIAFID ORCID Logo 
Pages
296-301
Section
Original Article
Publication year
2019
Publication date
Mar 2019
Publisher
Mashhad University of Medical Sciences
ISSN
20083866
e-ISSN
20083874
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.22038/ijbms.2019.31611.7611
ProQuest document ID
2381679842
Copyright
© 2019. This work is published under https://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.