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© 2016. This article is published under (http://creativecommons.org/licenses/by-nc-sa/3.0/) (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Our preliminary proteomics analysis suggested that expression of microtubule-associated protein tau is elevated in the spinal cord after injury. Therefore, the first aim of the present study was to examine tau expression in the injured spinal cord. The second aim was to determine whether tau can regulate neural stem cell migration, a critical factor in the successful treatment of spinal cord injury. We established rat models of spinal cord injury and injected them with mouse hippocampal neural stem cells through the tail vein. We used immunohistochemistry to show that the expression of tau protein and the number of migrated neural stem cells were markedly increased in the injured spinal cord. Furthermore, using a Transwell assay, we showed that neural stem cell migration was not affected by an elevated tau concentration in the outer chamber, but it was decreased by changes in intracellular tau phosphorylation state. These results demonstrate that neural stem cells have targeted migration capability at the site of injury, and that although tau is not a chemokine for targeted migration of neural stem cells, intracellular tau phosphorylation/dephosphorylation can inhibit cell migration.

Details

Title
Effects of microtubule-associated protein tau expression on neural stem cell migration after spinal cord injury
Author
Zhi-ping Qi 1 ; Guo-xiang, Wang 1 ; Xia, Peng 1 ; Ting-ting Hou 1 ; Hong-li, Zhou 2 ; Wang, Tie-jun 1 ; Xiao-yu, Yang 1 

 Department of Orthopedics, the Second Hospital of Jilin University, Changchun, Jilin Province 
 Department of Orthopedics, China-Japan Union Hospital of Jilin University, Changchun, Jilin Province 
Pages
332-337
Publication year
2016
Publication date
Feb 2016
Publisher
Medknow Publications & Media Pvt. Ltd.
ISSN
16735374
e-ISSN
18767958
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2382724008
Copyright
© 2016. This article is published under (http://creativecommons.org/licenses/by-nc-sa/3.0/) (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.