Mechanical properties of nerve roots and rami radiculares isolated from fresh pig spinal cords

Abstract

No reports have described experiments designed to determine the strength characteristics of spinal nerve roots and rami radiculares for the purpose of explaining the complexity of symptoms of medullary cone lesions and cauda equina syndrome. In this study, to explain the pathogenesis of cauda equina syndrome, monoaxial tensile tests were performed to determine the strength characteristics of spinal nerve roots and rami radiculares, and analysis was conducted to evaluate the stress-strain relationship and strength characteristics. Using the same tensile test device, the nerve root and ramus radiculares isolated from the spinal cords of pigs were subjected to the tensile test and stress relaxation test at load strain rates of 0.1, 1, 10, and 100 s ^-1 under identical settings. The tensile strength of the nerve root was not rate dependent, while the ramus radiculares tensile strength tended to decrease as the strain rate increased. These findings provide important insights into cauda equina symptoms, radiculopathy, and clinical symptoms of the medullary cone.

Introduction

The cauda equina consists of nerve roots of the second and subsequent lumbar nerves joining within the spinal canal. Cauda equina syndrome is caused by compressive lesions, such as disc hernia and trauma, presenting with dyskinesia, sensory disorders, and bladder and rectal disorders. Furthermore, compression due to lesions of the medullary cone can also cause symptoms of myelopathy. To clarify the pathophysiology of cauda equina syndrome, morphological and histological evaluations have been conducted, involving preparation of models of chronic nerve root compression, traction of nerve roots, and other techniques (Jancalek et al., 2007; Singh et al., 2015). To date, however, no reports have described experiments designed to determine the strength characteristics of nerve roots and rami radiculares for the purpose of explaining the complexity of symptoms of medullary cone lesions and cauda equina syndrome.

In this study, we conducted a monoaxial tensile test using the nerve roots and rami radiculares isolated from fresh pig spinal cords to analyze the stress-strain relationships and strength characteristics.

Materials and Methods

Tensile test machine

The same testing machine was used for all measurements in this study. The speed and dislocation were set using a personal computer (Windows XP Home Edition, One Microsoft Way Redmond, WA 98052-7329, USA and BTO, Naniwa, Osaka, Japan), and the pulling rate was controlled with a servo controller (SRCD10+RGU-2 Yamaha; Iwata, Shizuoka, Japan). The mobile part attached to the actuator was driven by the servo motor (T9H 20BK-400 Yamaha; Iwata, Shizuoka, Japan). The data for dislocation of the mobile part were collected with the rotary encoder attached to the servo motor and fed through the encoder board (PCI-6204 Interface; Kyobashi, Minami, Hiroshima, Japan) into the personal computer. The load data for the test samples were collected with the load cell (LTS-100GA Kyowa; Cyofugaoka, Cyofu, Tokyo, Japan) and fed through a dynamic strain meter (DPM-700B Kyowa; Cyofugaoka, Cyofu, Tokyo, Japan) and an AD conversion board (PCI-3171A Interface; Kyobashi, Minami, Hiroshima, Japan) into the personal computer [Figure 1]A, B).{Figure 1}

The program for controlling the measurements with the testing device was prepared with Microsoft Visual Basic 6.0 (One Microsoft Way Redmond, WA 98052-7329, USA). This program was used for setting the conditions for measurement (sampling frequency, pulling rate, tensile dislocation, and etc.) and processing of the data.

Preparation of nerve root samples

Samples were collected from the spinal cords of two pigs within 12 hours after slaughter for human consumption. Two pigs were hybrid (Landrace and Duroc), aged 13 months, weighing 120-130 kg, both genders (Ginchiku ranch company, Hofu, Yamaguchi, Japan). The pigs used in this experiment were dismantled for meat to eat. As confirmed by Science Research Center, Institute of Life Science and Medicine of Yamaguchi University, Japan, the precise procedure was unnecessary. The vertebral arch on the dorsal side of the spinal canal was resected with an electric saw (MJ-300, RYOBI) to expose the dura mater and the spinal cord [Figure 2]A). The nerve root was then dissected at the level of the intervertebral foramen, and the medullary cone and nerve root were isolated from the spine while still wrapped with the dura mater [Figure 2]B). The dura mater around the spinal cord and the nerve root were then resected [Figure 3]A), and samples 1-4 were assigned to the nerve root sections (sample No. 1 was assigned to the nerve root section most proximal to the point of bifurcation). During this process, the dura mater at the end of the nerve root facing the intervertebral foramen was left partially unresected to enable subsequent fixation on the testing device. For a similar reason, the spinal cord tissue at the origin of the nerve root was left partially unresected [Figure 3]B).

The sample was fixed in the testing device [Figure 1]B) by pinching the central and peripheral sides of the nerve root between the upper and lower jigs. To prevent the sample from falling off, the sample was fixed by suturing with 4-0 looped nylon using the method of Tsuge (1977).{Figure 2}{Figure 3}

Preparation of ramus radiculares samples

The specimens were immersed in physiological saline to enable ramus radiculares samples to be prepared without damaging the specimen [Figure 3]B). The diameter of each ramus radiculares sample was measured with a 3D laser microscope (LEXT OLS4000; Olympus, Japan), followed by calculation of the cross-sectional area. A mean cross-section of 0.069 mm 2 (range: 0.067-0.071 mm 2 ) was adopted. To analyze the mechanical strength characteristics of the nerve roots and constituent rami radiculares, samples were subjected to the tensile test and to the stress relaxation test at a gauge length of 10 mm and temperature of 25°C as reported earlier (Ichihara et al., 2001). The load strain rates were 0.1, 1, 10 and 100 s -1 using 3, 4, 3 and 4 samples of nerve roots and 4, 6, 6, and 5 samples of ramus radiculares, respectively. We used Microsoft Office Home and Business 2010 Excel (One Microsoft Way Redmond, WA 98052-7329, USA) for preparing figures.

Results

Nerve root tensile test results

To analyze the mechanical properties of nerve roots following compression by trauma or because of degeneration of the disc or vertebral body, we conducted tensile tests. [Figure 4]A shows the stress-strain relationships for nerve roots analyzed using monoaxial tensile tests. With each sample, the stress gradually increased towards its maximum as the strain increased, thus exhibiting a superelastic pattern. After reaching its maximum value, the stress began to decrease gradually even as the strain increased further. {Figure 4}

Rate dependency of nerve root

To investigate damage to the nerve root caused by speed, we further evaluated the results of the tensile test. [Figure 4]B shows the data for nerve root tensile strength (σB ) in relation to the load strain rate (ε). When ε = 1.0 s -1 the relationship between σB and ε remained relatively constant, despite large variations in these parameters, and the tensile strength was not rate dependent. At each strain rate, the nerve root σB was approximately 0.7 MPa.

Ramus radiculares tensile test results

To examine differences between the nerve root and ramus radiculares, we conducted similar tensile tests to investigate the mechanical properties of ramus radiculares. [Figure 5]A-D shows stress-strain relationships using ramus radiculares samples. Although differences in ε did not influence deformation patterns, the response to stress of the ramus radiculares differed markedly to that of nerve roots. When ε = 0.01 s -1 the stress decreased in a stepwise manner after reaching a peak, with the decrease becoming more rapid as the strain rate increased.{Figure 5}

Rate dependency of ramus radiculares tensile strength

To examine the effect of speed on damage to the ramus radiculares, we further evaluated the results of tensile testing. [Figure 6] shows results for the mean tensile strength (σB ) of all ramus radiculares samples in relation to the load strain rate (ε). The tensile strength of the ramus radiculares tended to decrease as the load strain rate increased.{Figure 6}

Discussion

The pathophysiology and classification of lumbar spinal canal stenosis were first reported by Arnoldi (1976). After this initial report, many researchers performed clinical and animal studies examining the symptoms of cauda equina in the context of blood flow (Ooi et al., 1990; Takahashi et al., 1993; Ikawa et al., 2005) and dural pressure both in the standing position and at rest (Takahashi et al., 1995a, b). According to these reports, the dural pressure during walking or in the standing position is approximately twice that in the supine position, and the disruption of circulation in patients with cauda equina syndrome is attributable to venous retention rather than to insufficient arterial blood supply.

Furthermore, for clarification of the pathophysiology of compressive radiculopathy, experiments on acute compression (Delamarter et al., 1990; Olmarker et al., 1991; Kobayashi et al., 1993) and chronic compression (Kobayashi et al., 2004a, b) have been carried out to evaluate nerve degeneration and pathological changes. In addition, evaluation of nerve root traction and compression has also been conducted frequently. Singh et al. (2015) reported that the morphological changes in nerve roots vary depending on the type of external force. Moreover, Jancalek et al. (2007) conducted histological evaluations of the internal structures of nerves exposed to external force. Reports have also described studies of molecules involved in pain, such as tumor necrosis factor (TNF)-α during nerve root compression (Obata et al., 2004; Sekiguchi et al., 2009). These experiments were designed to create a similar environment of entrapment neuropathy by applying a constant pressure to the nerve.

While previous reports have evaluated blood flow or pressure, histological features or degeneration, and the pathology/chemistry, no studies have examined the mechanical properties of nerve roots or rami radiculares. When discussing the occurrence of paralysis arising from compression or trauma, mechanical properties also need to be taken into consideration. Although previous studies investigated the pathological and chemical changes of nerve roots following compression, they did not evaluate damage to the nerve caused by force and speed of injury. While this can be described with respect to future changes in the nerve, it is not possible to estimate the degree of nerve damage or to predict the effects of speed and traction. Our analysis is capable of shedding light on this. For example, after trauma to the thoracolumbar vertebral junction, symptoms are diverse because this area is governed by the upper and lower motor neurons of the spinal cord parenchyma, cauda equina, and nerve roots, thus making diagnosis difficult (Elsberg, 1996). The symptoms in these tissues may be associated with the strength characteristics of the cauda equina, in addition to the anatomical complexity and the manner of compression (Wall et al., 1990a, b). For these reasons, we attempted tensile tests to clarify the mechanical properties of these tissues.

A similar evaluation of the strength characteristics of nerves was previously reported by Ichihara et al. (2001) who analyzed the material characteristics of the spinal cord (gray and white matter). Based on data describing these mechanical properties, simulation by the finite element method has been conducted for analysis of the features of spinal cord disease, yielding results that were very similar to clinical knowledge (Kato et al., 2008, 2009, 2010; Nishida et al., 2011, 2012, 2013, 2014). In the present study, the nerve root tensile strength showed no rate dependency and was about 0.7 MPa; this value is 16 times the gray matter strength and 47 times the white matter strength reported by Ichihara et al. (2001). These results indicated that the nerve root has higher tensile strength than the spinal cord. This feature may be associated with the observation that severe injury of the nerve root in the medullary cone is unlikely to occur. Regarding the rami radiculares, the present study suggested the possibility that the nerve may be injured suddenly when exposed to certain stress. As the pulling rate increased, the likelihood for injury tended to increase as well. Additionally, the stepwise decrease in stress in the nerve root, unlike that in the ramus radiculares, seemed to be attributable to the composite structure of the nerve root, which is comprised of multiple rami, connective tissue, and fat tissue.

Since it is difficult to acquire human nerve root or ramus radiculares for study, we used tissues from the pig as an alternative. Clinical symptoms of cauda equina, radiculopathy and the medullary cone can be evaluated in relation to results obtained with the finite element method. If the finite element method analyses based on the mechanical properties of pig tissues correlate with the clinical symptoms of the patient, this may help to understand the underlying pathology and therefore help to choose the best therapeutic strategy.

This study had a few limitations. First, we did not perform evaluation of blood flow. Additionally, the pig specimens may have undergone histological degeneration during the 12-hour period from collection to examination. However, the finding that the nerve roots and rami radiculares had higher strength than the spinal cord may be important for analyzing the medullary cone and cauda equina and for understanding the clinical symptoms of diseases involving this areas.

Taken together, we performed tensile load tests in nerve roots and rami radiculares. The tensile strength of nerve roots was not rate dependent, while the tensile strength of rami radiculares tended to decrease as the strain rate increased. Our data showed that nerve roots and rami radiculares exhibited higher strength than the gray and white matter of the spinal cord. These findings provide important insight into the clinical symptoms of cauda equina syndrome and of diseases associated with the nerve roots and medullary cone.

Acknowledgments: Special thanks to Mr. Syota Tahara, Mr. Yoshitaka Kanda, Mr. Yuuta Haruna, Mr. Kunitaka Fukuda, Mr. Hidetaka Morita and Mr. Seiya Miyazaki who were cooperator and enforcer of the experiments.

Author contributions: NN and JO wrote the paper. JO and KI performed experiments. TK, XC, and TT guided the research. All authors approved the final version of this paper.[27]

Conflicts of interest: None declared.

Plagiarism check: This paper was screened twice using CrossCheck to verify originality before publication.

Peer review: This paper was double-blinded, stringently reviewed by international expert reviewers.