Full text

Turn on search term navigation

© 2015. This article is published under (http://creativecommons.org/licenses/by-nc-sa/3.0/) (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Post-stroke depression is associated with reduced expression of brain-derived neurotrophic factor (BDNF). In this study, we evaluated whether BDNF overexpression affects depression-like behavior in a rat model of post-stroke depression. The middle cerebral artery was occluded to produce a model of focal cerebral ischemia. These rats were then subjected to isolation-housing combined with chronic unpredictable mild stress to generate a model of post-stroke depression. A BDNF gene lentiviral vector was injected into the hippocampus. At 7 days after injection, western blot assay and real-time quantitative PCR revealed that BDNF expression in the hippocampus was increased in depressive rats injected with BDNF lentivirus compared with depressive rats injected with control vector. Furthermore, sucrose solution consumption was higher, and horizontal and vertical movement scores were increased in the open field test in these rats as well. These findings suggest that BDNF overexpression in the hippocampus of post-stroke depressive rats alleviates depression-like behaviors.

Details

Title
Overexpression of brain-derived neurotrophic factor in the hippocampus protects against post-stroke depression
Author
Hao-hao, Chen 1 ; Zhang, Ning 1 ; Wei-yun, Li 2 ; Ma-rong, Fang 2 ; Zhang, Hui 3 ; Yuan-shu, Fang 3 ; Ming-xing, Ding 1 ; Xiao-yan, Fu 1 

 Medical Molecular Biology Laboratory, School of Medicine, Jinhua Polytechnic, Jinhua, Zhejiang Province 
 Institute of Neuroscience, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province 
 Center of Experimental Animals, Jinhua Food and Drug Administration, Jinhua, Zhejiang Province 
Pages
1427-1432
Publication year
2015
Publication date
Sep 2015
Publisher
Medknow Publications & Media Pvt. Ltd.
ISSN
16735374
e-ISSN
18767958
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2382750112
Copyright
© 2015. This article is published under (http://creativecommons.org/licenses/by-nc-sa/3.0/) (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.