Abstract

Over the past two decades, many studies have shown that the yam storage protein dioscorin, which is abundant in the wastewater of starch processing, exhibits many biological activities both in vitro and in vivo. In the present study, the acid-precipitation method was optimized using Box-Behnken design (BBD) combined with response surface methodology (RSM) for the recovery of yam soluble protein (YSP) from wastewater. The experimental yield of YSP reached 57.7%. According to relative quantitative proteomics (LC-MS/MS), the crude YSP was mainly composed of 15 dioscorin isoforms, which was further verified by anion-exchange and size-exclusion chromatography. YSP was found to be rich in glutamic acid and aspartic acid, and the eight essential acids made up approximately 33.7% of the YSP. Moreover, the YSP demonstrated antioxidant activity, including scavenging DPPH, hydroxyl and superoxide anion radicals, and the possible structure-activity relationships were discussed. These results indicated that YSP produced by acid precipitation may be used as a protein source with antioxidant properties.

Details

Title
Recovery of Yam Soluble Protein from Yam Starch Processing Wastewater
Author
Heng-Yue, Xue 1 ; Zhao, Yue 2 ; Zi-Heng, Liu 2 ; Xiao-Wen, Wang 2 ; Jun-Wei, Zhang 2 ; Xue, Peng 2 ; Tanokura Masaru 3   VIAFID ORCID Logo  ; You-Lin, Xue 2   VIAFID ORCID Logo 

1  Liaoning University, College of Light Industry, Shenyang, P. R. China (GRID:grid.411356.4) (ISNI:0000 0000 9339 3042); Dalian Institute for Drug Control, Dalian, P.R. China (GRID:grid.411356.4) 
2  Liaoning University, College of Light Industry, Shenyang, P. R. China (GRID:grid.411356.4) (ISNI:0000 0000 9339 3042) 
3  The University of Tokyo, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, Tokyo, Japan (GRID:grid.26999.3d) (ISNI:0000 0001 2151 536X) 
Publication year
2020
Publication date
2020
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-020-62372-6
ProQuest document ID
2382998164
Copyright
This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.