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Copyright © 2020, Nuñez-Rivera et al.; licensee Beilstein-Institut. This work is published under https://creativecommons.org/licenses/by/4.0 (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

There is an increasing interest in the use of plant viruses as vehicles for anti-cancer therapy. In particular, the plant virus brome mosaic virus (BMV) and cowpea chlorotic mottle virus (CCMV) are novel potential nanocarriers for different therapies in nanomedicine. In this work, BMV and CCMV were loaded with a fluorophore and assayed on breast tumor cells. The viruses BMV and CCMV were internalized into breast tumor cells. Both viruses, BMV and CCMV, did not show cytotoxic effects on tumor cells in vitro. However, only BMV did not activate macrophages in vitro. This suggests that BMV is less immunogenic and may be a potential carrier for therapy delivery in tumor cells. Furthermore, BMV virus-like particles (VLPs) were efficiently loaded with small interfering RNA (siRNA) without packaging signal. The gene silencing was demonstrated by VLPs loaded with siGFP and tested on breast tumor cells that constitutively express the green fluorescent protein (GPF). After VLP-siGFP treatment, GFP expression was efficiently inhibited corroborating the cargo release inside tumor cells and the gene silencing. In addition, BMV VLP carring siAkt1 inhibited the tumor growth in mice. These results show the attractive potential of plant virus VLPs to deliver molecular therapy to tumor cells with low immunogenic response.

Details

Title
Brome mosaic virus-like particles as siRNA nanocarriers for biomedical purposes
Author
Nuñez-Rivera, Alfredo; Fournier Pierrick G J; Arellano, Danna L; Rodriguez-Hernandez, Ana G; Vazquez-Duhalt, Rafael; Cadena-Nava, Ruben D
University/institution
U.S. National Institutes of Health/National Library of Medicine
Pages
372-382
Publication year
2020
Publication date
2020
Publisher
Beilstein-Institut zur Föerderung der Chemischen Wissenschaften
e-ISSN
21904286
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2384657286
Copyright
Copyright © 2020, Nuñez-Rivera et al.; licensee Beilstein-Institut. This work is published under https://creativecommons.org/licenses/by/4.0 (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.