Genome-wide quantitative trait loci mapping on Verticillium wilt resistance in 300 chromosome segment substitution lines from Gossypium hirsutum × Gossypium barbadense

Abstract

Abstract

Cotton Verticillium wilt (VW) is a devastating disease seriously affecting fiber yield and quality, and the most effective and economical prevention measure at present is selection and extension of Gossypium varieties harboring high resistant VW. However, multiple attempts to improve the VW resistance of the most widely cultivated Upland cotton have brought in little significant progress, and it seems necessary and urgent to develop Chromosome segment substitution lines (CSSLs) for merging the superior genes related with high yield and wide adaptation from G. hirsutum and VW resistance and excellent fiber quality from G. barbadense. In this study, 300 CSSLs were chosen from the developed BC₅F_3:5 CSSLs constructed by G. hirsutum CCRI36 and G. barbadense Hai1 to conduct quantitative trait locus (QTL) mapping on VW resistance, and a total of 53 QTLs relevant to VW disease index (DI) were identified together with the phenotypic data of 2 years investigations in two fields with two replications per year. All the QTLs were distributed on 20 chromosomes with phenotypic variation of 3.74-11.89%, of which 29 stable ones were consistent in at least two environments. Based on Meta-analysis on the 53 QTLs, 43 novel ones were identified, while 10 ones consistent to previously identified QTLs. Meanwhile, 32 QTL hotspot regions were detected, including 15 ones were novel. This study concentrates on QTL identification and screening hotspot region related with VW in the 300 CSSLs, which lay a solid platform not only for revealing the genetic and molecular mechanisms of VW resistance, but also for further fine mapping, gene cloning and molecular designing in breeding program for resistant cotton varieties.

Footnotes

* Md Harun or Rashid: harunbjri{at}yahoo.com, Peng-tao Li: lipengtao1056{at}126.com, Tingting Chen: chentingting7039{at}126.com, Koffi Kibalou Palanga: palangaeddieh{at}yahoo.fr, Wan-kui Gong: wkgong{at}aliyun.com, Qun Ge: gequn{at}caas.cn, Ju-wu Gong: gongjuwu{at}caas.cn, Ai-ying Liu: liuaiying{at}caas.cn, Quan-wei Lu: 13707667581{at}163.com, Latyr Diouf: latyr{at}cricaas.com.cn, Zareen Sarfraz: zareensarfraz88{at}ciit.net.pk, Muhammad Jamshed: jamshed_muhammad{at}yahoo.com, Yu-zhen Shi: shiyuzhen{at}caas.cn, You-lu Yuan: yuanyoulu{at}caas.cn

*

Abbreviations

English Abbr.

English Full Name

VW

Verticillium Wilt

DI

Disease Index

SSR

Simple Sequence Repeats

CSSL

Chromosome Segment Substitution Lines

AIC

Akaike Information Criterion

CI

Confidence Interval

Chr

Chromosome

cM

Centi-Morgan

CMD

Cotton Marker Database

H²_B

Broad sense Heritability

LOD

Logarithm of Odds

MAS

Marker Assisted Selection

QTL

Quantitative Trait Loci

PV

Phenotypic Variation

CTAB

Cetyl-Trimethyl Ammonium Bromide

Mya

Million Years Ago