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© 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Objective

To identify potential microRNA (miRNA) biomarkers of poor, normal and hyperresponse to controlled ovarian stimulation (COS).

Methods

In the present study, we assessed 40 serum samples from patients undergoing COS. We used ten samples to standardize miRNAs detection in the serum. The remaining 30 samples were split into three groups depending on the patient's response to COS: poor response (PR group, n=10), normal response (NR group, n=10), and hyperresponse (HR group, n=10). Aberrantly expressed miRNAs were identified using a large-scale expression analysis platform. Gene set enrichment analysis was performed to assess the biological processes potentially modulated by the identified miRNAs.

Results

Twenty-two miRNAs were detected only in the PR or HR groups when compared with the NR group. From those, 11 presented poor dissociation curves and were excluded from further analysis. A bioinformatics analysis revealed that the selected 11 miRNAs target several genes involved in GnRH, estrogen and prolactin signaling, oocyte maturation, female pregnancy, and meiosis.

Conclusion

The large-scale analysis of miRNA expression identified distinct miRNA profiles for poor and hyperresponse to COS, which potentially modulate key processes for human assisted reproduction. All evidence suggests that the serum microRNA profiling may discriminate patients who will respond in an exacerbated manner from those who will respond insufficiently to COS. Further studies may validate these miRNAs, enabling the individualization of treatment and more successful outcomes.

Details

Title
Serum microRNA profiling for the identification of predictive molecular markers of the response to controlled ovarian stimulation
Author
Borges Jr. Edson; Mulato Maria Gabriela Ferreira; Souza, Setti Amanda; Assumpto, Iaconelli Jr; Vieira, Geraldo Murilo; Paes de Almeida Ferreira Braga Daniela
University/institution
U.S. National Institutes of Health/National Library of Medicine
Pages
97-103
Publication year
2020
Publication date
2020
Publisher
Sociedade Brasileira de Reprodução Humana (Brazilian Society of Assisted Reproduction)
ISSN
15175693
e-ISSN
15180557
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2396849629
Copyright
© 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.