Abstract
A need was sensed to provide positive control to all laboratories in the national network of Viral Research and Diagnostic Laboratories (VRDLs) for real-time RT-PCR. [...]positive controls for the screening and confirmatory assays were generated in-house. [...]an IVT RNA was designed for entire N gene which would be compatible for multiple protocols. [...]gene-specific IVT RNA was synthesized for all the gene targets used in real time PCR.
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Details
1 Influenza Group, ICMR-National Institute of Virology, Pune 411 001, Maharashtra
2 Electron Microscopy & Pathology Group, ICMR-National Institute of Virology, Pune 411 001, Maharashtra
3 Bioinformatics & Data Management Group, ICMR-National Institute of Virology, Pune 411 001, Maharashtra
4 ICMR-National Institute of Virology, Pune 411 001, Maharashtra