Oxidative Stress in Plants

Abstract

The high concentrations of these reactive species that exceed the capacity of antioxidant defence enzymes, disturb redox homeostasis, which could trigger damage to macromolecules, such as membrane lipids, proteins and nucleic acids, and ultimately result in nitro-oxidative stress and plant cell death. [...]they proposed that Tyr73 would be a possible residue to be involved in reducing this enzymatic activity. [...]Dr. Rivero’s group [3] investigated the response of tomato plants to the effects of calcium and potassium on plant tolerance to combined high-temperature and salinity stress conditions. [6] provided up-to-date information about the response and function of ROS and RNS, mainly with regard to superoxide radicals, hydrogen peroxide and nitric oxide under drought stress conditions, and their scavenging by the antioxidant defence enzymes in several plant species. In summary, to better understand the nitro-oxidative stress networks in higher plants (Figure 1), the subjects addressed in this special issue provide an update and new knowledge about ROS and RNS metabolisms in plant responses to adverse environmental stimuli and the modulation of antioxidant systems to control ROS production and accumulation.

Environmental stresses negatively affect plant growth, development and crop productivity. These adverse conditions alter the metabolism of reactive oxygen and nitrogen species (ROS and RNS, respectively). The high concentrations of these reactive species that exceed the capacity of antioxidant defence enzymes, disturb redox homeostasis, which could trigger damage to macromolecules, such as membrane lipids, proteins and nucleic acids, and ultimately result in nitro-oxidative stress and plant cell death. Significant progress has been made to understand how plants persist in these stressful environments that could be vital to improve plant crop yield. In this special issue “Oxidative Stress in Plants”, both original articles and detailed reviews have been published with the aim to provide an up-date view in this research area in higher plants.

In the natural environment, plants are constantly exposed to abiotic stresses, such as extreme temperatures, salt stress, drought and heavy metals that have a huge impact on agriculture worldwide and consequently, lead to massive economic losses. In this sense, three research papers have analysed the effect of abiotic stress on plant growth and development. Dr. Wani’s group [1] studied the role of Serratia marcescens BM1 in response to cadmium (Cd) stress in soybean plants by different physiological, biochemical and molecular assays. They found that, in Cd-stressed plants, the Serratia marcescens BM1 treatment not only down-regulated Cd levels and oxidative stress markers, but also up-regulated levels of osmolytes, stress-related gene expression and activities of antioxidant enzymes. These authors suggested that inoculation with the Serratia marcescens BM1 would promotes Cd stress tolerance and phytoremediation potential. The impact of abiotic stress was also reported by Dr. Barroso’s group [2] as they demonstrated the effect of short-term low temperature stress on the metabolism of reactive oxygen and nitrogen species in Arabidopsis plants. These authors showed that the low temperature produces nitro-oxidative stress, and reduces cytosolic NADP-malic enzyme activity, which was negatively modulated by the protein tyrosine nitration process. In addition, they proposed that Tyr73 would be a possible residue to be involved in reducing this enzymatic activity. Moreover, Dr. Rivero’s group [3] investigated the response of tomato plants to the effects of calcium and potassium on plant tolerance to combined high-temperature and salinity stress conditions. They showed the positive effect of a rise in calcium and potassium in the nutrient medium on the improvement of oxidative stress produced under these environmental stress injuries. The authors underlined the importance of the correctly administering of nutrient solution and fertilisation to face the damaging effects of adverse conditions in plant cells.

On the other hand, plant cells develop an antioxidant defence mechanism, which includes the non-enzymatic and enzymatic antioxidants for the detoxification of ROS. However, if the ROS production is higher than the ability of the antioxidant systems to scavenge them, it can lead to oxidative stress, and finally to cell death. In this context, Dr. De Maio’s group [4] used citrus plants to investigate the modulation of poly (ADP-ribose) polymerase and antioxidant enzymes, using leaves in different developmental stages, including young, mature and senescent. Their work addressed the physiological, biochemical and molecular changes that occur in plant cells during leaf ageing. In young leaves, photochemical and glutathione-S-transferase activities increased. However, while the ageing process advanced, the non-enzymatic antioxidant systems reduced and reached the lowest levels in senescent leaves, while poly (ADP-ribose) polymerase activity increased. In the same way, Hasanuzzaman et al. [5] discussed in an extensive review, the available and up-to-date knowledge on the Ascorbate-Glutathione pathway concerning the oxidative stress tolerance, as well as plant defence mechanisms. Furthermore, the review by Laxa et al. [6] provided up-to-date information about the response and function of ROS and RNS, mainly with regard to superoxide radicals, hydrogen peroxide and nitric oxide under drought stress conditions, and their scavenging by the antioxidant defence enzymes in several plant species. To better understand the interaction between chitosan and Vitis vinifera L. plants, the original article by Singh et al. [7] analysed the antioxidant potential, the total phenolic content and the expression of ROS detoxification genes in two red grapevine varieties treated by chitosan. They concluded that chitosan induced the phenolic compounds, as well as acted as the organiser for the transfer of polyphenols from the Vitis vinifera leaves to the berries.

Another interesting feature of this special issue focuses on investigating the other H₂O₂ targets involved in programmed cell death. Dr. Mano’s group [8] studied the mechanism that increased the reactive carbonyl species in the H₂O₂-produced programmed cell death in tobacco Bright Yellow-2 cells. They suggested that H₂O₂ initially inactivates a carbonyl reductase(s), which increases the reactive carbonyl species content, leading to the activation of the caspase-3-like protease of the 20S proteasome. The authors proposed that carbonyl reductase acted as a ROS sensor for inducing programmed cell death.

In plant cells, the ROS metabolism has been widely studied in different compartments, including mitochondria, cytosol, chloroplast, cell wall, plasma membrane, apoplast, glyoxysomes and peroxisomes [9]. The review by Dr. Petřivalský’s group [10] provided the present knowledge about the compartment-specific pathways of reactive oxygen species generation and decomposition in plant cells, and the mechanisms that controlling their homeostasis in cell compartments. Likewise, with a particular example at the chloroplastic level, in an in-depth review Miyake [11] summarised the current research concerning the molecular mechanisms of ROS formation and suppression in photosystem I. He established a novel molecular mechanism for the oxidation of the P700 oxidation system in photosystem I and the elimination of ROS formation from the strong relationship between the light and dark reactions of photosynthesis. Furthermore, in an original article, Lewandowska et al. [12] investigated the effect of H₂O₂ on the structure and function of Arabidopsis chloroplastic DJ-1B. They found that AtDJ-1B has double functions, namely holdase and glyoxalase activity, which responded differently to H₂O₂. Glyoxalase activity was reduced by H₂O₂, however the holdase chaperone function did not change. They also analysed the phenotype of T-DNA lines that lacked the protein, and showed that AtDJ-1B was not necessary for plant growth under stress stimuli.

In summary, to better understand the nitro-oxidative stress networks in higher plants (Figure 1), the subjects addressed in this special issue provide an update and new knowledge about ROS and RNS metabolisms in plant responses to adverse environmental stimuli and the modulation of antioxidant systems to control ROS production and accumulation.

Figure 1. Schematic model of cross-talk between reactive oxygen species (ROS) and reactive nitrogen species (RNS) in plant responses to abiotic stress. Different abiotic stress situations can generate an uncontrolled production of ROS and RNS that oxidatively modify different biomolecules (proteins, lipids and nucleic acids). These modifications can lead to a gain of function of the antioxidant systems to control the production of ROS or generate a situation of cellular damage supported by a process of nitro-oxidative stress. The numbers indicate the relationship of each article in the Special Issue to the subject matter covered. (1) El-Esawi et al., 2020 [1]. (2) Begara-Morales et al., 2019 [2]. (3) García-Martí et al., 2019 [3]. (4) Biswas et al., 2020 [8]. (5) Janků et al., 2019 [10]. (6) Miyake, 2020 [11]. (7) Lewandowska et al., 2019 [12]. (8) Arena et al., 2019 [4]. (9) Hasanuzzaman et al., 2019 [5]. (10) Laxa et al., 2019 [6]. (11) Singh et al., 2019 [7].

Funding

This research was funded by ERDF grants co-financed by the Ministry of Economy and Competitiveness (project PGC2018-096405-B-I00), the Junta de Andalucía (group BIO286), the action 6 of the Research Support Plan of the University of Jaén for (2017-2019) R08/06/2019 and the R + D + I project within the framework Program of FEDER Andalucía 2014-2020 (Reference: 1263509).

Conflicts of Interest

The authors declare no conflict of interest.

References

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