Abstract

Here, we describe a new immersion-based clearing method suitable for optical clearing of thick adult human brain samples while preserving its lipids and lipophilic labels such as 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI). This clearing procedure is simple, easy to implement, and allowed for clearing of 5 mm thick human brain tissue samples within 12 days. Furthermore, we show for the first time the advantageous effect of the Periodate-Lysine-Paraformaldehyde (PLP) fixation as compared to the more commonly used 4% paraformaldehyde (PFA) on clearing performance.

Details

Title
hFRUIT: An optimized agent for optical clearing of DiI-stained adult human brain tissue
Author
Hildebrand Sven 1   VIAFID ORCID Logo  ; Schueth, Anna 1 ; Wangenheim Klaus von 2 ; Mattheyer Christian 3 ; Pampaloni, Francesco 3 ; Bratzke Hansjürgen 4 ; Roebroeck, Alard F 1 ; Galuske Ralf A W 2 

1  Department of Cognitive Neuroscience, Faculty of Psychology & Neuroscience, Maastricht University, Maastricht, the Netherlands (GRID:grid.5012.6) (ISNI:0000 0001 0481 6099) 
2  Department of Systemic Neurophysiology, Technische Universität Darmstadt, Darmstadt, Germany (GRID:grid.6546.1) (ISNI:0000 0001 0940 1669) 
3  Johann Wolfgang Goethe-Universität Frankfurt am Main, Faculty 15 Biological Sciences, Institute for Cell Biology and Neuroscience, Buchmann Institute for Molecular Life Sciences, Frankfurt am Main, Germany (GRID:grid.7839.5) (ISNI:0000 0004 1936 9721) 
4  Johann Wolfgang Goethe-Universität Frankfurt am Main, Institute for Forensic Medicine, Frankfurt am Main, Germany (GRID:grid.7839.5) (ISNI:0000 0004 1936 9721) 
Publication year
2020
Publication date
2020
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-020-66999-3
ProQuest document ID
2414910021
Copyright
© The Author(s) 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.