Abstract

Inadequate nutrient intake leads to oxidative stress disrupting homeostasis, activating signaling, and altering metabolism. Oxidative stress serves as a hallmark in developing prostate lesions, and an aggressive cancer phenotype activating mechanisms allowing cancer cells to adapt and survive. It is unclear how adaptation and survival are facilitated; however, literature across several organisms demonstrates that a reversible cellular growth arrest and the transcription factor, nuclear factor-kappaB (NF-κB), contribute to cancer cell survival and therapeutic resistance under oxidative stress. We examined adaptability and survival to oxidative stress following nutrient deprivation in three prostate cancer models displaying varying degrees of tumorigenicity. We observed that reducing serum (starved) induced reactive oxygen species which provided an early oxidative stress environment and allowed cells to confer adaptability to increased oxidative stress (H2O2). Measurement of cell viability demonstrated a low death profile in stressed cells (starved + H2O2), while cell proliferation was stagnant. Quantitative measurement of apoptosis showed no significant cell death in stressed cells suggesting an adaptive mechanism to tolerate oxidative stress. Stressed cells also presented a quiescent phenotype, correlating with NF-κB nuclear translocation, suggesting a mechanism of tolerance. Our data suggests that nutrient deprivation primes prostate cancer cells for adaptability to oxidative stress and/or a general survival mechanism to anti-tumorigenic agents.

Details

Title
Serum deprivation initiates adaptation and survival to oxidative stress in prostate cancer cells
Author
White, ElShaddai Z 1 ; Pennant, Nakea M 1 ; Carter, Jada R 1 ; Hawsawi Ohuod 2 ; Odero-Marah Valerie 1 ; Hinton, Cimona V 1 

 Clark Atlanta University (CAU), Department of Biological Sciences, Atlanta, USA (GRID:grid.254275.3) (ISNI:0000 0001 2224 3669); Clark Atlanta University (CAU), Center for Cancer Research and Therapeutic Development, Atlanta, USA (GRID:grid.254275.3) (ISNI:0000 0001 2224 3669) 
 Baylor College of Medicine, Department of Molecular and Human Genetics, Houston, USA (GRID:grid.39382.33) (ISNI:0000 0001 2160 926X) 
Publication year
2020
Publication date
2020
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2427393319
Copyright
© The Author(s) 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.