Abstract

Optical coherence tomography offers astounding opportunities to image the complex structure of living tissue but lacks functional information. We present dynamic full-field optical coherence tomography as a technique to noninvasively image living human induced pluripotent stem cell-derived retinal organoids. Coloured images with an endogenous contrast linked to organelle motility are generated, with submicrometre spatial resolution and millisecond temporal resolution, creating a way to identify specific cell types in living tissue via their function.

Details

Title
Dynamic full-field optical coherence tomography: 3D live-imaging of retinal organoids
Author
Scholler Jules 1   VIAFID ORCID Logo  ; Groux Kassandra 1 ; Goureau Olivier 2 ; José-Alain, Sahel 3 ; Fink, Mathias 1   VIAFID ORCID Logo  ; Reichman Sacha 2   VIAFID ORCID Logo  ; Boccara, Claude 1 ; Grieve, Kate 4 

1  PSL University, CNRS, Institut Langevin, ESPCI Paris, Paris, France (GRID:grid.4444.0) (ISNI:0000 0001 2112 9282) 
2  Sorbonne Université, INSERM, CNRS, Institut de la Vision, Paris, France (GRID:grid.4444.0) 
3  Sorbonne Université, INSERM, CNRS, Institut de la Vision, Paris, France (GRID:grid.4444.0); Quinze-Vingts National Eye Hospital, Paris, France (GRID:grid.415610.7) (ISNI:0000 0001 0657 9752); Fondation Ophtalmologique Rothschild, Paris, France (GRID:grid.417888.a) (ISNI:0000 0001 2177 525X); The University of Pittsburgh School of Medicine, Department of Ophthalmology, Pittsburgh, United States (GRID:grid.21925.3d) (ISNI:0000 0004 1936 9000) 
4  Sorbonne Université, INSERM, CNRS, Institut de la Vision, Paris, France (GRID:grid.4444.0); Quinze-Vingts National Eye Hospital, Paris, France (GRID:grid.415610.7) (ISNI:0000 0001 0657 9752) 
Publication year
2020
Publication date
2020
Publisher
Springer Nature B.V.
e-ISSN
20477538
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41377-020-00375-8
ProQuest document ID
2434615974
Copyright
© The Author(s) 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.