Preimplantation genetic diagnosis: the GATA experience

Abstract

In this study, results of genetic analysis, success rates of implantation, pregnancy and live birth of 18 patients in whom preimplantation genetic diagnosis was performed by making biopsy in their 3rd day embriyos with 6-8 blastomers at the Assisted Reproductive Techniques Unit of Department of Obstetrics and Gynecology of Gülhane Military Medical Faculty between September 2005 and June 2007 were evaluated. Genetic analysis was performed by fluorescent in situ hybridization method on the same day. According to the results of genetic analysis and embryonic growth, embryos with a number of 1 to 3 were transferred. Two hundred and nine oocytes were collected during in vitro fertilization procedures of 18 patients. Of these oocytes, intracytoplasmic sperm injection procedure was performed to 183, which were mature, and 127 embriyos were achieved. Blastomere biopsy was performed to 83 of these embryos which had grade I quality. When the patients were analyzed according to the indications of preimplantation genetic diagnosis, 1, 11, 5 and 1 patients had a history of chromosomal anomaly, recurrent implantation failure, recurrent abortus and cryptozoospermia, respectively. Of the 16 patients performed embryo transfer after preimplantation genetic diagnosis, 8 (50%) and 5 (31.3%) patients had chemical and clinical pregnancy, respectively. In view of the results of the present study preimplantation genetic diagnosis seems to be an effective method in in vitro diagnosis of chromosomal anomalies of couples who have a risk of genetic disease and/or recurrent implantation failure.