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© 2020. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Extensively drug-resistant (XDR) Salmonella Typhi has been reported in Sindh province of Pakistan since 2016. The potential for further spread is of serious concern as remaining treatment options are severely limited. We report the phenotypic and genotypic characterization of 27 XDR S. Typhi isolated from patients attending Jinnah Hospital, Lahore, Pakistan. Isolates were identified by biochemical profiling; antimicrobial susceptibility was determined by a modified Kirby–Bauer method. These findings were confirmed using Illumina whole genome nucleotide sequence data. All sequences were compared to the outbreak strain from Southern Pakistan and typed using the S. Typhi genotyping scheme. All isolates were confirmed by a sequence analysis to harbor an IncY plasmid and the CTX-M-15 ceftriaxone resistance determinant. All isolates were of the same genotypic background as the outbreak strain from Sindh province. We report the first emergence of XDR S. Typhi in Punjab province of Pakistan confirmed by whole genome sequencing.

Details

Title
Emergence of Resistance to Fluoroquinolones and Third-Generation Cephalosporins in Salmonella Typhi in Lahore, Pakistan
Author
Rasheed, Farhan  VIAFID ORCID Logo  ; Saeed, Muhammad; Nabil-Fareed Alikhan; Baker, David; Khurshid, Mohsin  VIAFID ORCID Logo  ; Ainsworth, Emma V  VIAFID ORCID Logo  ; Turner, A Keith; Ambereen Anwar Imran; Muhammad Hidayat Rasool; Saqalein, Muhammad; Muhammad Atif Nisar; Muhammad Fayyaz ur Rehman  VIAFID ORCID Logo  ; Wain, John; Muhammad Yasir; Langridge, Gemma C  VIAFID ORCID Logo  ; Ikram, Aamer  VIAFID ORCID Logo 
First page
1336
Publication year
2020
Publication date
2020
Publisher
MDPI AG
e-ISSN
20762607
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2440408824
Copyright
© 2020. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.