Abstract

Fine-tuning quantitative traits for continuous subtle phenotypes is highly advantageous. We engineer the highly conserved upstream open reading frame (uORF) of FvebZIPs1.1 in strawberry (Fragaria vesca), using base editor A3A-PBE. Seven novel alleles are generated. Sugar content of the homozygous T1 mutant lines is 33.9–83.6% higher than that of the wild-type. We also recover a series of transgene-free mutants with 35 novel genotypes containing a continuum of sugar content. All the novel genotypes could be immediately fixed in subsequent generations by asexual reproduction. Genome editing coupled with asexual reproduction offers tremendous opportunities for quantitative trait improvement.

Details

Title
Fine-tuning sugar content in strawberry
Author
Xing, Sinian; Chen, Kunling; Zhu, Haocheng; Zhang, Rui; Zhang, Huawei; Li, Bingbing; Gao, Caixia  VIAFID ORCID Logo 
Pages
1-14
Section
Method
Publication year
2020
Publication date
2020
Publisher
BioMed Central
ISSN
14747596
e-ISSN
1474760X
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1186/s13059-020-02146-5
ProQuest document ID
2444112206
Copyright
© 2020. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.