Full text

Turn on search term navigation

Copyright © 2020 Fereshteh Beigmohammadi et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0/

Abstract

Genetic studies have illustrated that killer cell immunoglobulin-like receptor (KIR) genes could participate in various autoimmune disorders. We aimed to clarify the role of KIR genes, HLA ligands, HLA-KIR interactions, and their genotypes in inflammatory bowel disease (IBD) susceptibility. The study population was composed of 183 IBD subjects, comprising 100 ulcerative colitis (UC) patients, 83 Crohn’s disease (CD) patients, and 274 healthy subjects. Polymerase chain reaction with sequence-specific primers (PCR-SSP) was used to evaluate the absence or presence of the 15 KIR genes, 5 HLA class I ligands, and 2 pseudogenes. We did not find any significant difference in allele frequency of KIRs and pseudogenes between IBD patients and healthy controls. In the case of HLA genes, there was a significant difference in HLA-B-Bw4Thr80 frequency between UC patients and healthy controls (P=0.03, OR=0.06, 95%CI=0.008-0.4). Furthermore, we found a significant difference in HLA-C1Asn80 frequency between CD patients and healthy controls (P=0.04, OR=0.49, 95% CI=0.3-0.8). In the full-array combination of KIR genes, there was no significant frequency difference between UC patients and healthy controls, while two KIR genotypes showed a significant susceptible association with CD. Our data do not support a strong role of NK cells in IBD susceptibility, but it does not rule out a role for KIR variability in IBD patients. However, there are some protective associations such as Bw4 alleles; these associations may be due to the interaction of the alleles to TCRs rather than KIRs.

Details

Title
Analysis of Killer Cell Immunoglobulin-Like Receptor Genes and Their HLA Ligands in Inflammatory Bowel Diseases
Author
Beigmohammadi, Fereshteh 1 ; Mahmoudi, Mahdi 2   VIAFID ORCID Logo  ; Karami, Jafar 3 ; Ahmadzadeh, Nooshin 1 ; Ebrahimi-Daryani, Nasser 4 ; Rezaei, Nima 5   VIAFID ORCID Logo 

 Rheumatology Research Center, Tehran University of Medical Sciences, Tehran, Iran 
 Rheumatology Research Center, Tehran University of Medical Sciences, Tehran, Iran; Rheumatology Expert Group (REG), Universal Scientific Education and Research Network (USERN), Tehran, Iran; Inflammation Research Center, Tehran University of Medical Sciences, Tehran, Iran 
 Rheumatology Research Center, Tehran University of Medical Sciences, Tehran, Iran; Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran 
 Department of Gastroenterology and Hepatology, Tehran University of Medical Sciences, Tehran, Iran 
 Research Center for Immunodeficiencies, Children’s Medical Center, Tehran University of Medical Sciences, Tehran, Iran; Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran; Network of Immunity in Infection, Malignancy and Autoimmunity (NIIMA), Universal Scientific Education and Research Network (USERN), Tehran, Iran 
Editor
Patrice Petit
Publication year
2020
Publication date
2020
Publisher
John Wiley & Sons, Inc.
ISSN
23148861
e-ISSN
23147156
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2446479592
Copyright
Copyright © 2020 Fereshteh Beigmohammadi et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0/