Full text

Turn on search term navigation

© 2020. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Acorn barnacles are major marine fouling organisms. Their success is largely due to an ability to adhere to diverse substrates via a sub-micron thick proteinaceous adhesive layer that develops as the organism molts and expands its base. Recent work has expanded the set of proteins identified within the adhesive interface, but one outstanding question concerns their spatial distribution throughout the organism. Here, we employ immunological analysis of Amphibalanus amphitrite tissue sections and identify the presence of two cement proteins, AaCP19-1 and AaCP43-1, in areas far removed from the adhesive interface. Confocal imaging reveals specific staining along different tissue linings of the organism as well as other non-cementing regions. Additionally, we employ a modified, pressure cycling technology approach to recover protein from histological tissue sections to perform proteomics analysis. Mass spectrometry analysis of proteins recovered from transverse histological sections of the upper portion of barnacles indicates the presence of these same proteins, complementing the immunostaining observations. The proteomics analysis also revealed the presence of other proteins first identified in the adhesive layer. While some proteins are clearly enriched at the surface interface, our findings challenge the concept that cement proteins are exclusive to the substrate interface and suggest they may have an expanded physiological role beyond substrate adhesion-related processes of A. amphitrite.

Details

Title
Distribution of Select Cement Proteins in the Acorn Barnacle Amphibalanus amphitrite
Author
Schultzhaus, Janna N; Wang, Chenyue; Patel, Shrey; Smerchansky, Madeline; Phillips, Daniel; Taitt, Chris R; Leary, Dagmar H; Hervey, Judson; Dickinson, Gary H; So, Christopher R; Scancella, Jenifer M; Wahl, Kathryn J; Spillmann, Christopher M
Section
Original Research ARTICLE
Publication year
2020
Publication date
Oct 6, 2020
Publisher
Frontiers Research Foundation
e-ISSN
2296-7745
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2448726267
Copyright
© 2020. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.