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Abstract
The aim of the present study was to evaluate the effects of temperature, substrate, and luminosity on chia seeds in order to determine adequate conditions for a germination test. The experiment consisted of a completely randomized design, in a 4 × 5 × 2 factorial scheme (four substrates × five temperatures × two luminosity conditions), with four replications. The following variables were analyzed: first germination count, germination, germination speed index, and mean germination time. Germination was best at 15, 20, 25, and 30 °C for the on-paper, paper roll, and sand substrates. However, in the presence of luminosity and at 25 °C, the on-paper substrate had the highest mean. For all substrates, the lowest germination was achieved at 35 °C, proving that high temperatures reduce chia seed germination. Germination speed index was highest at 25 and 30 °C for on-paper, paper roll, and sand substrates, in both the presence and absence of luminosity. Chia seeds are considered to be neutral photoblasts. Germination tests should be performed with the on-paper substrate, at 25 °C, and with a duration of seven days. The first count should be carried out four days after establishment of the test.