Abstract

This study represents the green synthesis of silver nanoparticles (AgNPs) using Ipomoea aquatica (Water Spinach) leaf extract, and investigate the cytotoxicity and antimicrobial activities. The Ipomoea aquatica (IA) mediated green synthesized AgNPs (IA-AgNPs) was characterized by ultraviolet–visible (UV–VIS.) spectrophotometry, Fourier transform-infrared (FT-IR) spectroscopy, atomic absorption spectrometry (AAS), X-ray deffractometry (XRD), transmission electron microscopy (TEM), and energy dispersive X-ray (EDX) spectroscopy. The results showed that the material was formed in nano-dimension with particle size in the range between 15–30 nm. The nanoparticles (NPs) were almost spherical in shape and demonstrated excellent material stability. The PC12 cell model in-vitro was used to determine the cytotoxicity of the IA-AgNPs, and for antimicrobial study, E. coli, Salmonella, and Staphylococcus sp. were employed. The cell viability results showed that the NPs did not induce any cytotoxicity until 10 ppm for 48 h of incubation, and it was further confirmed by the lactate dehydrogenase (LDH) assay. Moreover, IA-AgNPs demonstrated good antibacterial activity against three bacteria: E. coli, Salmonella, and Staphylococcus sp. Thus, this study may create an important synthetic route for the production of green-AgNPs and their safe usages, and may also be used to augment the bioactivities of the material with minimum toxicity.

Details

Title
Green synthesis of silver nanoparticles using Ipomoea aquatica leaf extract and its cytotoxicity and antibacterial activity assay
Author
Khan, Md Rokonujaman 1 ; Hoque, Sheikh Manjura 2 ; Kaniz Fatima Binte Hossain 3   VIAFID ORCID Logo  ; Md Abu Bakar Siddique 4 ; Md Khabir Uddin 5   VIAFID ORCID Logo  ; Rahman, Md Mostafizur 5 

 Department of Environmental Sciences, Jahangirnagar University, Dhaka, Bangladesh; Bangladesh National Authority for Chemical Weapons Convention, Dhaka Cantonment, Dhaka, Bangladesh 
 Materials Science Division, Atomic Energy Centre, Dhaka, Bangladesh 
 Graduate School of Environmental Science, Hokkaido University, Sapporo, Japan 
 Institute of National Analytical Research and Service (INARS), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka, Bangladesh 
 Department of Environmental Sciences, Jahangirnagar University, Dhaka, Bangladesh 
Pages
303-315
Publication year
2020
Publication date
Dec 2020
Publisher
Taylor & Francis Ltd.
ISSN
17518253
e-ISSN
17517192
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2468554459
Copyright
© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This work is licensed under the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.