Abstract

In Holsteins, emerging evidence suggests that long non-coding RNAs (lncRNAs) are actively involved in the regulation of mammary gland development. However, the lncRNA expression pattern in the early growth stage of Holstein mammary gland remains little known. In this study, we comprehensively analyzed 11 mammary gland tissue RNA-Seq datasets derived from Holstein heifer calves (ca. 8 weeks old) retrieved from the Sequence Read Archive database. We identified a total of 588 lncRNAs in the intergenic region. By comparing the sequences with NONCODEv5 database, 241 novel lncRNAs were detected in the mammary gland. Prediction of cis-regulated target gene revealed that 364 out of 588 lncRNAs potentially targeted 417 protein-coding genes. Furthermore, Gene Ontology enrichment determined that potential target genes were mainly involved in the terms associated with energy metabolism and cell differentiation, such as cellular respiration, respiratory electron transport chain, electron transport chain, and regulation of cell differentiation, which were closely related to mammary gland development. This study provides potential candidate lncRNAs in regulating mammary gland development in the early growth stage of Holsteins, as well as a better general understanding of Holstein mammary gland development.

Details

Title
Identification of long non-coding RNAs in the early growth stage of Holstein mammary gland
Author
K Du 1 ; A-Y, Ren 1 ; M-C, Cai 2   VIAFID ORCID Logo  ; G-Z, Wang 3 ; Jia, X-B 1 ; S-Q Hu 1   VIAFID ORCID Logo  ; Wang, J 1 ; S-Y, Chen 1 ; S-J, Lai 1 

1  Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China 
2  Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China; College of Landscape Architecture and Life Science/Institute of Special Plants, Chongqing University of Arts and Sciences, Chongqing, China 
3  Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China; The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang, China 
Pages
214-222
Publication year
2020
Publication date
Dec 2020
Publisher
Taylor & Francis Ltd.
ISSN
24750255
e-ISSN
24750263
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1080/24750263.2020.1747557
ProQuest document ID
2469501330
Copyright
© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This work is licensed under the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.