Abstract

Contact with stinging spines venom from several Lepidoptera larvae may result in skin lesions. In Mexico, envenomation outbreaks caused by Megalopyge opercularis were reported between 2015 and 2016. The aim of this study was to identify the venomous caterpillars in Nuevo Leon, Mexico and evaluate several biological activities of their hemolymph (HEV) and spine setae (SSV) venoms. M. opercularis was identified by cytochrome oxidase subunit (COI) designed primers. HEV and SSV extracts cytotoxic activity was assessed on the L5178Y-R lymphoma cell line. For apoptotic cells number and apoptosis, cells were stained with acridine orange/ethidium bromide and validated by DNA fragmentation. Human peripheral blood mononuclear cells (hPBMC) cytokine response to the extracts was measured by the cytometric bead array assay. Extracts effect on pro-coagulation activity on human plasma was also evaluated. HEV and SSV extracts significantly inhibited (p < 0.01) up to 63% L5178Y-R tumor cell growth at 125–500 µg/mL, as compared with 43% of Vincristine. About 79% extracts-treated tumor cells death was caused by apoptosis. Extracts stimulated (p < 0.01) up to 60% proliferation of resident murine lymphocytes, upregulated IL-1β, IL-6, IL-8, and TNF-α production by hPBMC, and showed potent pro-coagulant effects. The pharmacological relevance of these venoms is discussed.

Details

Title
In vitro antitumor, pro-inflammatory, and pro-coagulant activities of Megalopyge opercularis J.E. Smith hemolymph and spine venom
Author
Orozco-Flores, Alonso A 1   VIAFID ORCID Logo  ; Valadez-Lira, José A 1   VIAFID ORCID Logo  ; Covarrubias-Cárdenas, Karina E 1   VIAFID ORCID Logo  ; Pérez-Trujillo, José J 2   VIAFID ORCID Logo  ; Gomez-Flores, Ricardo 1   VIAFID ORCID Logo  ; Caballero-Hernández, Diana 1   VIAFID ORCID Logo  ; Tamez-Guerra Reyes 1   VIAFID ORCID Logo  ; Rodríguez-Padilla, Cristina 1   VIAFID ORCID Logo  ; Tamez-Guerra, Patricia 1   VIAFID ORCID Logo 

 Universidad Autónoma de Nuevo León (UANL), Departamento de Microbiología E Inmunología, Facultad de Ciencias Biológicas (FCB), San Nicolás de los Garza, Mexico (GRID:grid.411455.0) (ISNI:0000 0001 2203 0321) 
 UANL, Departamento de Histología, Facultad de Medicina, Monterrey, Mexico (GRID:grid.411455.0) (ISNI:0000 0001 2203 0321) 
Publication year
2020
Publication date
2020
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2471523441
Copyright
© The Author(s) 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.