Abstract

Cell division can perturb the metabolic performance of industrial microbes. The C period of cell division starts from the initiation to the termination of DNA replication, whereas the D period is the bacterial division process. Here, we first shorten the C and D periods of E. coli by controlling the expression of the ribonucleotide reductase NrdAB and division proteins FtsZA through blue light and near-infrared light activation, respectively. It increases the specific surface area to 3.7 μm−1 and acetoin titer to 67.2 g·L−1. Next, we prolong the C and D periods of E. coli by regulating the expression of the ribonucleotide reductase NrdA and division protein inhibitor SulA through blue light activation-repression and near-infrared (NIR) light activation, respectively. It improves the cell volume to 52.6 μm3 and poly(lactate-co-3-hydroxybutyrate) titer to 14.31 g·L−1. Thus, the optogenetic-based cell division regulation strategy can improve the efficiency of microbial cell factories.

Manipulation of genes controlling microbial shapes can affect bio-production. Here, the authors employ an optogenetic method to realize dynamic morphological engineering of E. coli replication and division and show the increased production of acetoin and poly(lactate-co-3-hydroxybutyrate).

Details

Title
Light-powered Escherichia coli cell division for chemical production
Author
Ding Qiang 1 ; Ma Danlei 1 ; Gao-Qiang, Liu 2   VIAFID ORCID Logo  ; Yang, Li 1 ; Guo, Liang 1 ; Gao Cong 1 ; Hu Guipeng 1 ; Ye, Chao 1 ; Liu, Jia 1 ; Liu, Liming 3 ; Chen Xiulai 1   VIAFID ORCID Logo 

1  Jiangnan University, State Key Laboratory of Food Science and Technology, Wuxi, China (GRID:grid.258151.a) (ISNI:0000 0001 0708 1323); Jiangnan University, Key Laboratory of Industrial Biotechnology, Ministry of Education, Wuxi, China (GRID:grid.258151.a) (ISNI:0000 0001 0708 1323) 
2  Central South University of Forestry and Technology, Hunan Provincial Key Laboratory for Forestry Biotechnology, Changsha, China (GRID:grid.440660.0) (ISNI:0000 0004 1761 0083) 
3  Jiangnan University, State Key Laboratory of Food Science and Technology, Wuxi, China (GRID:grid.258151.a) (ISNI:0000 0001 0708 1323); Jiangnan University, Key Laboratory of Industrial Biotechnology, Ministry of Education, Wuxi, China (GRID:grid.258151.a) (ISNI:0000 0001 0708 1323); Jiangnan University, National Engineering Laboratory for Cereal Fermentation Technology, Wuxi, China (GRID:grid.258151.a) (ISNI:0000 0001 0708 1323) 
Publication year
2020
Publication date
2020
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41467-020-16154-3
ProQuest document ID
2471540951
Copyright
© The Author(s) 2020. corrected publication 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.