Abstract

Glucagon-Like Peptide-1 (GLP-1) undergoes rapid inactivation by dipeptidyl peptidase-4 (DPP4) suggesting that target receptors may be activated by locally produced GLP-1. Here we describe GLP-1 positive cells in the rat and human stomach and found these cells co-expressing ghrelin or somatostatin and able to secrete active GLP-1 in the rats. In lean rats, a gastric load of glucose induces a rapid and parallel rise in GLP-1 levels in both the gastric and the portal veins. This rise in portal GLP-1 levels was abrogated in HFD obese rats but restored after vertical sleeve gastrectomy (VSG) surgery. Finally, obese rats and individuals operated on Roux-en-Y gastric bypass and SG display a new gastric mucosa phenotype with hyperplasia of the mucus neck cells concomitant with increased density of GLP-1 positive cells. This report brings to light the contribution of gastric GLP-1 expressing cells that undergo plasticity changes after bariatric surgeries, to circulating GLP-1 levels.

GLP-1 is a gastrointestinal peptide that regulates gastric acid secretion and emptying, and due to the rapid degradation of intestinally secreted GLP-1 local gastric production has been suggested. Here the authors report the presence of GLP-1 expressing cells in the rat and human stomach, which contribute to the circulating GLP-1 levels and are affected by weight loss surgeries.

Details

Title
Bariatric surgery induces a new gastric mucosa phenotype with increased functional glucagon-like peptide-1 expressing cells
Author
Ribeiro-Parenti, Lara 1 ; Anne-Charlotte, Jarry 2 ; Cavin Jean-Baptiste 2 ; Willemetz Alexandra 2 ; Le, Beyec Johanne 3 ; Sannier Aurélie 4 ; Benadda Samira 5 ; Pelletier Anne-Laure 6 ; Hourseau Muriel 4 ; Léger Thibaut 7   VIAFID ORCID Logo  ; Morlet Bastien 8 ; Couvelard, Anne 9 ; Anini Younes 10 ; Msika Simon 11 ; Marmuse Jean-Pierre 11 ; Ledoux Sévérine 12 ; Le Gall Maude 6   VIAFID ORCID Logo  ; Bado André 6   VIAFID ORCID Logo 

1  Université de Paris, Inserm U1149, Centre de Recherche sur l’inflammation, Paris, France; Service de Chirurgie Générale Œsogastrique et Bariatrique, Hôpital Bichat - Claude-Bernard, Assistance Publique-Hôpitaux de Paris, Paris, France 
2  Université de Paris, Inserm U1149, Centre de Recherche sur l’inflammation, Paris, France 
3  Université de Paris, Inserm U1149, Centre de Recherche sur l’inflammation, Paris, France; Sorbonne Université, AP-HP, Hôpital Pitié-Salpêtrière-Charles Foix, Biochimie Endocrinienne et Oncologique, Paris, France 
4  Department of Pathology Bichat Hospital, AP-HP, Paris, France (GRID:grid.50550.35) (ISNI:0000 0001 2175 4109) 
5  Université de Paris, Inserm U1149, Centre de Recherche sur l’inflammation, Paris, France (GRID:grid.50550.35); Cell and Tissue Imaging Platform, Inserm, U1149, CNRS, ERL8252, Paris, France (GRID:grid.50550.35) 
6  Université de Paris, Inserm U1149, Centre de Recherche sur l’inflammation, Paris, France (GRID:grid.50550.35) 
7  Université de Paris, Mass Spectrometry Laboratory, Institut Jacques Monod, UMR 7592, CNRS, Paris, France (GRID:grid.50550.35); Université Rennes, Inserm, EHESP, Irset (Institut de recherche en santé, environnement et travail) - UMR_S 1085, Rennes, France (GRID:grid.410368.8) (ISNI:0000 0001 2191 9284) 
8  Université de Paris, Mass Spectrometry Laboratory, Institut Jacques Monod, UMR 7592, CNRS, Paris, France (GRID:grid.410368.8) 
9  Université de Paris, Inserm U1149, Centre de Recherche sur l’inflammation, Paris, France (GRID:grid.410368.8); Department of Pathology Bichat Hospital, AP-HP, Paris, France (GRID:grid.50550.35) (ISNI:0000 0001 2175 4109) 
10  Dalhousie University, IWK Health Centre, Department of Obstetrics and Gynecology, Halifax, Canada (GRID:grid.55602.34) (ISNI:0000 0004 1936 8200) 
11  Université de Paris, Inserm U1149, Centre de Recherche sur l’inflammation, Paris, France (GRID:grid.55602.34); Service de Chirurgie Générale Œsogastrique et Bariatrique, Hôpital Bichat - Claude-Bernard, Assistance Publique-Hôpitaux de Paris, Paris, France (GRID:grid.55602.34) 
12  Université de Paris, Inserm U1149, Centre de Recherche sur l’inflammation, Paris, France (GRID:grid.55602.34); Service des Explorations Fonctionnelles Hôpital Louis Mourier, AP-HP, Centre Intégré Nord Francilien de prise en charge de l’Obésité (CINFO), Colombes, France (GRID:grid.50550.35) (ISNI:0000 0001 2175 4109) 
Publication year
2021
Publication date
2021
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41467-020-20301-1
ProQuest document ID
2474981520
Copyright
© The Author(s) 2021. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.