It appears you don't have support to open PDFs in this web browser. To view this file, Open with your PDF reader
Abstract
Chinese hamster ovary (CHO) cells have been the most commonly used mammalian host for large-scale commercial production of therapeutic proteins, such as monoclonal antibodies. Enhancement of productivity of these CHO cells is one of the top priorities in the biopharmaceutical industry to reduce manufacturing cost. Although there are many different methods (e.g. temperature, pH, feed) to improve protein production in CHO cells, the role of physiologically relevant hydrostatic pressure in CHO cell culture has not been reported yet. In this study, four different hydrostatic pressures (0, 30, 60, and 90 mmHg) were applied to batch CHO cells, and their cell growth/metabolism and IgG1 production were examined. Our results indicate that hydrostatic pressure can increase the maximum cell concentration by up to 50%. Moreover, overall IgG1 concentration on Day 5 showed that 30 mmHg pressure can increase IgG1 production by 26%. The percentage of non-disulphide-linked antibody aggregates had no significant change under pressure. Besides, no significant difference was observed between 30 mmHg and no pressure conditions in terms of cell clumping formation. All these findings are important for the optimization of fed-batch or perfusion culture for directing cell growth and improving antibody production.
You have requested "on-the-fly" machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Show full disclaimer
Neither ProQuest nor its licensors make any representations or warranties with respect to the translations. The translations are automatically generated "AS IS" and "AS AVAILABLE" and are not retained in our systems. PROQUEST AND ITS LICENSORS SPECIFICALLY DISCLAIM ANY AND ALL EXPRESS OR IMPLIED WARRANTIES, INCLUDING WITHOUT LIMITATION, ANY WARRANTIES FOR AVAILABILITY, ACCURACY, TIMELINESS, COMPLETENESS, NON-INFRINGMENT, MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. Your use of the translations is subject to all use restrictions contained in your Electronic Products License Agreement and by using the translation functionality you agree to forgo any and all claims against ProQuest or its licensors for your use of the translation functionality and any output derived there from. Hide full disclaimer
Details
1 Singapore-MIT Alliance for Research and Technology (SMART) Centre, Critical Analytics for Manufacturing Personalized-Medicine (CAMP) IRG, Singapore, Singapore (GRID:grid.429485.6) (ISNI:0000 0004 0442 4521); National University of Singapore, Department of Biomedical Engineering, Singapore, Singapore (GRID:grid.4280.e) (ISNI:0000 0001 2180 6431)
2 Massachusetts Institute of Technology, Research Laboratory of Electronics, Cambridge, USA (GRID:grid.116068.8) (ISNI:0000 0001 2341 2786)
3 Massachusetts Institute of Technology, Department of Chemical Engineering, Cambridge, USA (GRID:grid.116068.8) (ISNI:0000 0001 2341 2786)
4 Singapore-MIT Alliance for Research and Technology (SMART) Centre, Critical Analytics for Manufacturing Personalized-Medicine (CAMP) IRG, Singapore, Singapore (GRID:grid.429485.6) (ISNI:0000 0004 0442 4521); National University of Singapore, Mechanobiology Institute, Singapore, Singapore (GRID:grid.4280.e) (ISNI:0000 0001 2180 6431); National University of Singapore, Department of Biomedical Engineering, Singapore, Singapore (GRID:grid.4280.e) (ISNI:0000 0001 2180 6431); National University of Singapore, Department of Mechanical Engineering, Singapore, Singapore (GRID:grid.4280.e) (ISNI:0000 0001 2180 6431); Institute for Health Innovation and Technology, Singapore, Singapore (GRID:grid.4280.e)
5 City University of Hong Kong, Department of Biomedical Engineering, Kowloon Tong, Hong Kong (GRID:grid.35030.35) (ISNI:0000 0004 1792 6846)
6 Singapore-MIT Alliance for Research and Technology (SMART) Centre, Critical Analytics for Manufacturing Personalized-Medicine (CAMP) IRG, Singapore, Singapore (GRID:grid.429485.6) (ISNI:0000 0004 0442 4521); National University of Singapore, Department of Mechanical Engineering, Singapore, Singapore (GRID:grid.4280.e) (ISNI:0000 0001 2180 6431); Massachusetts Institute of Technology, Department of Electrical Engineering and Computer Science, Department of Biological Engineering, Cambridge, USA (GRID:grid.116068.8) (ISNI:0000 0001 2341 2786); Massachusetts Institute of Technology, Research Laboratory of Electronics, Cambridge, USA (GRID:grid.116068.8) (ISNI:0000 0001 2341 2786)