Abstract

Leaf angle is an important agronomic trait affecting photosynthesis efficiency and crop yield. Although the mechanisms involved in the leaf angle control are intensively studied in monocots, factors contribute to the leaf angle in dicots are largely unknown. In this article, we explored the physiological roles of an Arabidopsis glucosyltransferase, UGT74D1, which have been proved to be indole-3-acetic acid (IAA) glucosyltransferase in vitro. We found that UGT74D1 possessed the enzymatic activity toward IAA glucosylation in vivo and its expression was induced by auxins. The ectopically expressed UGT74D1 obviously reduced the leaf angle with an altered IAA level, auxin distribution and cell size in leaf tissues. The expression of several key genes involved in the leaf shaping and leaf positioning, including PHYTOCHROME KINASE SUBSTRATE (PKS) genes and TEOSINTE BRANCHED1, CYCLOIDEA, and PCF (TCP) genes, were dramatically changed by ectopic expression of UGT74D1. In addition, clear transcription changes of YUCCA genes and other auxin related genes can be observed in overexpression lines. Taken together, our data indicate that glucosyltransferase UGT74D1 could affect leaf positioning through modulating auxin homeostasis and regulating transcription of PKS and TCP genes, suggesting a potential new role of UGT74D1 in regulation of leaf angle in dicot Arabidopsis.

Details

Title
The ectopic expression of Arabidopsis glucosyltransferase UGT74D1 affects leaf positioning through modulating indole-3-acetic acid homeostasis
Author
Jin Shanghui 1 ; Hou Bingkai 2 ; Zhang Guizhi 3 

 Qingdao Agricultural University, College of Life Sciences, Qingdao, China (GRID:grid.412608.9) (ISNI:0000 0000 9526 6338); Shandong University, The Key Laboratory of Plant Development and Environment Adaptation Biology, Ministry of Education, College of Life Sciences, Qingdao, China (GRID:grid.27255.37) (ISNI:0000 0004 1761 1174) 
 Shandong University, The Key Laboratory of Plant Development and Environment Adaptation Biology, Ministry of Education, College of Life Sciences, Qingdao, China (GRID:grid.27255.37) (ISNI:0000 0004 1761 1174) 
 Linyi University, School of Pharmacy, College of Pharmacy, Linyi, China (GRID:grid.410747.1) (ISNI:0000 0004 1763 3680); Shandong University, The Key Laboratory of Plant Development and Environment Adaptation Biology, Ministry of Education, College of Life Sciences, Qingdao, China (GRID:grid.27255.37) (ISNI:0000 0004 1761 1174) 
Publication year
2021
Publication date
2021
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2477377718
Copyright
© The Author(s) 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.