Abstract

Nitrogen (N) and carbon (C) are essential elements for plant growth and crop yield. Thus, improved N and C utilisation contributes to agricultural productivity and reduces the need for fertilisation. In the present study, we find that overexpression of a single rice gene, Oryza sativa plasma membrane (PM) H+-ATPase 1 (OSA1), facilitates ammonium absorption and assimilation in roots and enhanced light-induced stomatal opening with higher photosynthesis rate in leaves. As a result, OSA1 overexpression in rice plants causes a 33% increase in grain yield and a 46% increase in N use efficiency overall. As PM H+-ATPase is highly conserved in plants, these findings indicate that the manipulation of PM H+-ATPase could cooperatively improve N and C utilisation, potentially providing a vital tool for food security and sustainable agriculture.

Improved utilisation of nitrogen and carbon could boost agricultural productivity. Here Zhang et al. show that overexpression of a single gene, encoding the plasma membrane H+ -ATPase 1 OSA1, is able to increase both carbon fixation via photosynthesis and nitrogen assimilation via ammonium uptake in rice.

Details

Title
Plasma membrane H+-ATPase overexpression increases rice yield via simultaneous enhancement of nutrient uptake and photosynthesis
Author
Zhang Maoxing 1 ; Wang, Yin 2 ; Chen, Xi 3 ; Xu Feiyun 4 ; Ding, Ming 4 ; Ye Wenxiu 5   VIAFID ORCID Logo  ; Kawai Yuya 6 ; Toda Yosuke 7   VIAFID ORCID Logo  ; Hayashi Yuki 6 ; Suzuki, Takamasa 8   VIAFID ORCID Logo  ; Zeng Houqing 9 ; Liang, Xiao 4 ; Xiao, Xin 10 ; Xu, Jin 11 ; Guo Shiwei 4 ; Feng, Yan 12 ; Shen Qirong 4   VIAFID ORCID Logo  ; Xu, Guohua 4   VIAFID ORCID Logo  ; Kinoshita Toshinori 13   VIAFID ORCID Logo  ; Zhu Yiyong 4   VIAFID ORCID Logo 

1  Nanjing Agricultural University, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, College of Resources and Environment Sciences, Nanjing, China (GRID:grid.27871.3b) (ISNI:0000 0000 9750 7019); Nagoya University, Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya, Japan (GRID:grid.27476.30) (ISNI:0000 0001 0943 978X) 
2  Nagoya University, Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya, Japan (GRID:grid.27476.30) (ISNI:0000 0001 0943 978X); Peking University, Institute of Ecology, College of Urban and Environmental Sciences and Key Laboratory for Earth Surface Processes of Ministry of Education, Beijing, China (GRID:grid.11135.37) (ISNI:0000 0001 2256 9319) 
3  Nanjing Agricultural University, College of Life Sciences, Nanjing, China (GRID:grid.27871.3b) (ISNI:0000 0000 9750 7019) 
4  Nanjing Agricultural University, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, College of Resources and Environment Sciences, Nanjing, China (GRID:grid.27871.3b) (ISNI:0000 0000 9750 7019) 
5  Nagoya University, Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya, Japan (GRID:grid.27476.30) (ISNI:0000 0001 0943 978X); Shanghai Jiao Tong University, School of Agriculture and Biology, Shanghai, China (GRID:grid.16821.3c) (ISNI:0000 0004 0368 8293) 
6  Nagoya University, Graduate School of Science, Nagoya, Japan (GRID:grid.27476.30) (ISNI:0000 0001 0943 978X) 
7  Nagoya University, Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya, Japan (GRID:grid.27476.30) (ISNI:0000 0001 0943 978X); PRESTO, Japan Science and Technology Agency (JST), Kawaguchi, Japan (GRID:grid.419082.6) (ISNI:0000 0004 1754 9200) 
8  Chubu University, Department of Biological Chemistry, College of Bioscience and Biotechnology, Kasugai, Japan (GRID:grid.254217.7) (ISNI:0000 0000 8868 2202) 
9  Hangzhou Normal University, College of Life and Environmental Sciences, Hangzhou, China (GRID:grid.410595.c) (ISNI:0000 0001 2230 9154) 
10  Anhui Science and Technology University, College of Resources and Environment, Fengyang, China (GRID:grid.443368.e) (ISNI:0000 0004 1761 4068) 
11  Shanxi Agricultural University, College of Horticulture, Taigu, China (GRID:grid.412545.3) (ISNI:0000 0004 1798 1300) 
12  Justus Liebig University, Institute of Agronomy and Plant Breeding, Giessen, Germany (GRID:grid.8664.c) (ISNI:0000 0001 2165 8627) 
13  Nagoya University, Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya, Japan (GRID:grid.27476.30) (ISNI:0000 0001 0943 978X); Nagoya University, Graduate School of Science, Nagoya, Japan (GRID:grid.27476.30) (ISNI:0000 0001 0943 978X) 
Publication year
2021
Publication date
2021
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41467-021-20964-4
ProQuest document ID
2485324993
Copyright
© The Author(s) 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.