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Abstract
Spectroscopic sensing provides physical and chemical information in a non-destructive and rapid manner. To develop non-destructive estimation methods of tea quality-related metabolites in fresh leaves, we estimated the contents of free amino acids, catechins, and caffeine in fresh tea leaves using visible to short-wave infrared hyperspectral reflectance data and machine learning algorithms. We acquired these data from approximately 200 new leaves with various status and then constructed the regression model in the combination of six spectral patterns with pre-processing and five algorithms. In most phenotypes, the combination of de-trending pre-processing and Cubist algorithms was robustly selected as the best combination in each round over 100 repetitions that were evaluated based on the ratio of performance to deviation (RPD) values. The mean RPD values were ranged from 1.1 to 2.7 and most of them were above the acceptable or accurate threshold (RPD = 1.4 or 2.0, respectively). Data-based sensitivity analysis identified the important hyperspectral regions around 1500 and 2000 nm. Present spectroscopic approaches indicate that most tea quality-related metabolites can be estimated non-destructively, and pre-processing techniques help to improve its accuracy.
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1 Shizuoka University, Faculty of Agriculture, Shizuoka, Japan (GRID:grid.263536.7) (ISNI:0000 0001 0656 4913); Gifu University, United Graduate School of Agricultural Science, Gifu, Japan (GRID:grid.256342.4) (ISNI:0000 0004 0370 4927)
2 Shizuoka University, Faculty of Agriculture, Shizuoka, Japan (GRID:grid.263536.7) (ISNI:0000 0001 0656 4913); Shizuoka University, Institute for Tea Science, Shizuoka, Japan (GRID:grid.263536.7) (ISNI:0000 0001 0656 4913)
3 Shizuoka University, Institute for Tea Science, Shizuoka, Japan (GRID:grid.263536.7) (ISNI:0000 0001 0656 4913); National Agriculture and Food Research Organization (NARO), Division of Tea Research, Institute of Fruit Tree and Tea Science, Shimada, Japan (GRID:grid.416835.d) (ISNI:0000 0001 2222 0432)