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Abstract
The role of microRNA-21-5p (miR-21-5p) in sepsis-induced acute kidney injury (AKI) has been seldom discussed. Therefore, the objective of this present study was to investigate the mechanism of endothelial progenitor cells-derived exosomes (EPCs-exos) in sepsis-induced AKI via miR-21-5p/runt-related transcription factor 1 (RUNX1) axis. miR-21-5p was downregulated and RUNX1 was upregulated in the kidney of cecal ligation and puncture (CLP) rats, and miR-21-5p targeted RUNX1. Elevation of miR-21-5p improved renal function and renal tissue pathological damage, attenuated serum inflammatory response, as well as reduced apoptosis and oxidative stress response in renal tissues, and regulated endothelial glycocalyx damage marker proteins syndecan-1 and heparanase-1 in CLP rats. Overexpression of RUNX1 abolished the impacts of elevated miR-21-5p in CLP rats. Also, EPCs-exos upregulated miR-21-5p expression, and functioned similar to elevation of miR-21-5p for CLP rats. Downregulating miR-21-5p partially reversed the effects of EPCs-exos on sepsis-induced AKI. Collectively, our study suggests that EPCs release miR-21-5p-containing exosomes to alleviate sepsis-induced AKI through RUNX1 silencing.
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1 Capital Medical University, Department of Nephrology, Beijing Friendship Hospital, Faculty of Kidney Diseases, Beijing, China (GRID:grid.24696.3f) (ISNI:0000 0004 0369 153X)
2 Capital Medical University, Department of Nephrology, Beijing Friendship Hospital, Faculty of Kidney Diseases, Beijing, China (GRID:grid.24696.3f) (ISNI:0000 0004 0369 153X); Capital Medical University, Center of Allergy, Beijing Shijitan Hospital, Beijing, China (GRID:grid.24696.3f) (ISNI:0000 0004 0369 153X)
3 Capital Medical University, Center of Allergy, Beijing Shijitan Hospital, Beijing, China (GRID:grid.24696.3f) (ISNI:0000 0004 0369 153X)
4 The University of Oklahoma Health Sciences Center, Department of Internal Medicine, College of Medicine, Oklahoma City, USA (GRID:grid.266902.9) (ISNI:0000 0001 2179 3618)
5 Vinnitsa National Medical University, Vinnitsa, Ukraine (GRID:grid.446037.2)