Abstract

Ideal methods for detecting pathogens should be sensitive, specific, rapid, cost-effective and instrument-free. Conventional nucleic acid pathogen detection strategies, mostly PCR-based techniques, have various limitations, such as expensive equipment, reagents and skilled performance. Recently, CRISPR/Cas-based methods have burst onto the scene, with the potential to power the pathogen detection field. Here we introduce these unique methods and discuss its hurdles and promises.

Details

Title
Next-generation pathogen diagnosis with CRISPR/Cas-based detection methods
Author
Wang, Xinjie 1   VIAFID ORCID Logo  ; Shang, Xiaoyun 2 ; Huang, Xingxu 3   VIAFID ORCID Logo 

1  Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, People’s Republic of China 
2  Suzhou Maximum Bio-tech Co., Ltd., Suzhou, People’s Republic of China 
3  School of Life Science and Technology, ShanghaiTech University, Shanghai, People’s Republic of China 
Pages
1682-1691
Publication year
2020
Publication date
Dec 2020
Publisher
Taylor & Francis Ltd.
e-ISSN
22221751
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1080/22221751.2020.1793689
ProQuest document ID
2508722280
Copyright
© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd. This work is licensed under the Creative Commons  Attribution – Non-Commercial License http://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.