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Abstract
The ability to characterize the combined structural, functional, and thermal properties of biophysically dynamic samples is needed to address critical questions related to tissue structure, physiological dynamics, and disease progression. Towards this, we have developed an imaging platform that enables multiple nonlinear imaging modalities to be combined with thermal imaging on a common sample. Here we demonstrate label-free multimodal imaging of live cells, excised tissues, and live rodent brain models. While potential applications of this technology are wide-ranging, we expect it to be especially useful in addressing biomedical research questions aimed at the biomolecular and biophysical properties of tissue and their physiology.
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Details
1 Vanderbilt University, Department of Biomedical Engineering, Nashville, USA (GRID:grid.152326.1) (ISNI:0000 0001 2264 7217)
2 Thorlabs Imaging Research, Sterling, USA (GRID:grid.152326.1)
3 Chongqing University, College of Bioengineering, Chongqing, China (GRID:grid.190737.b) (ISNI:0000 0001 0154 0904)
4 Thorlabs Imaging Research, Sterling, USA (GRID:grid.190737.b)
5 Vanderbilt University, Department of Biomedical Engineering, Nashville, USA (GRID:grid.152326.1) (ISNI:0000 0001 2264 7217); Vanderbilt University Medical Center, Department of Neurosurgery, Nashville, USA (GRID:grid.412807.8) (ISNI:0000 0004 1936 9916)